{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:59:47Z","timestamp":1781272787976,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T00:00:00Z","timestamp":1470614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Operational Programme \u201cCompetitiveness and Entrepreneurship\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>A simple and reliable high\u2010performance liquid chromatography method was developed and validated for the simultaneous determination of lipophilic antioxidants in tomato fruits using C<jats:sub>30<\/jats:sub> column operated at 15\u00b0C and a gradient mobile phase based on acetonitrile\/methanol\/dichloromethane in a total run time of 30 min. Diode array and fluorescence detectors were used respectively for the detection of carotenoids (lutein, zeaxanthin, cryptoxanthin, lycopene, and \u03b2\u2010carotene) and vitamin E analogs (\u03b1\u2010, \u03b2\u2010, \u03b3\u2010, and \u03b4\u2010tocopherols, and tocotrienols). The best extraction yield of analytes in tomato fruits was achieved by employing ethyl acetate\/hexane (1:1, v\/v) after several treatments with various solvents. In addition, low extraction yields were obtained for carotenoids compared to tocopherols by adopting solid\u2010phase extraction as a second clean\u2010up step. The method was validated on the basis of recovery, precision, linearity, and limit of detection and quantification using spiked tomato samples. The method was applied to cherry and medium\u2010sized tomato fruits. Lycopene was found to be present in largest amount in tomato pulp, followed by \u03b2\u2010carotene and lutein. Due to its simplicity, rapidity, and efficiency, the method is suitable for routine analysis of lipophilic antioxidants in tomato fruits, and may also be applied to other vegetables of similar phytochemical profiles.<\/jats:p>","DOI":"10.1002\/jssc.201600491","type":"journal-article","created":{"date-parts":[[2016,7,11]],"date-time":"2016-07-11T08:32:41Z","timestamp":1468225961000},"page":"3348-3356","source":"Crossref","is-referenced-by-count":20,"title":["Optimization and development of a high\u2010performance liquid chromatography method for the simultaneous determination of vitamin E and carotenoids in tomato fruits"],"prefix":"10.1002","volume":"39","author":[{"given":"Maria","family":"Irakli","sequence":"first","affiliation":[{"name":"Hellenic Agricultural Organization\u2013Demeter Plant Breeding and Genetic Resources Institute  Thermi Thessaloniki 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