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First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high\u2010speed counter\u2010counter chromatography using a two\u2010phase solvent system consisting of chloroform\/acetonitrile\/water (10:6:4, v\/v). The results showed high\u2010speed counter\u2010current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform\/methanol\/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from <jats:italic>Clematis akebioides<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201600805","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T02:56:12Z","timestamp":1476672972000},"page":"4660-4666","source":"Crossref","is-referenced-by-count":10,"title":["First separation of four aromatic acids and two analogues with similar structures and polarities from <i>Clematis akebioides<\/i> by high\u2010speed counter\u2010current chromatography"],"prefix":"10.1002","volume":"39","author":[{"given":"Chengcheng","family":"Gong","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering Southeast University  Nanjing P.R. 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