{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T22:01:27Z","timestamp":1766268087424,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T00:00:00Z","timestamp":1479427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Key Program of Natural Science Foundation of State","award":["81430093, 81373930, 81302905, 81202639, 81473584, 8167141849"],"award-info":[{"award-number":["81430093, 81373930, 81302905, 81202639, 81473584, 8167141849"]}]},{"name":"National Key Subject of Drug Innovation","award":["2015ZX09101043-005, 2015ZX09101043-011"],"award-info":[{"award-number":["2015ZX09101043-005, 2015ZX09101043-011"]}]},{"name":"TCM State Administration Subject of Public Welfare","award":["2015468004"],"award-info":[{"award-number":["2015468004"]}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province of China","doi-asserted-by":"crossref","award":["H2015038"],"award-info":[{"award-number":["H2015038"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province","award":["UNPYSCT-2015118"],"award-info":[{"award-number":["UNPYSCT-2015118"]}]},{"name":"Application Technology and Development of Youth Talents Project in Harbin","award":["2014RFQXJ116"],"award-info":[{"award-number":["2014RFQXJ116"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p><jats:italic>Zi Shen Wan<\/jats:italic> is a typical formula consisting of three herbs, <jats:italic>Phellodendri Amurensis Cortex<\/jats:italic>, <jats:italic>Rhizoma Anemarrhenae<\/jats:italic>, and <jats:italic>Cortex Cinnamomi<\/jats:italic>, and has been widely used for treating prostatitis and infection diseases. However, it lacks in\u2010depth research of the constituents of <jats:italic>Zi Shen Wan<\/jats:italic> in vivo and in vitro. In this work, ultra high performance liquid chromatography coupled with quadrupole\u2010time\u2010of\u2010flight mass spectrometry and MassLynx software was established to characterize the chemical compositions of <jats:italic>Zi Shen Wan<\/jats:italic> in vivo and in vitro. In total, 92 peaks were characterized in vitro and 33 peaks were characterized in vivo based on mass spectrometry and tandem mass spectrometry data. Among the 33 compounds characterized in rat plasma, 22 prototype components absorbed in rat serum and 11 metabolites were identified in vivo. This work was fully reports the chemical constituents of traditional Chinese formula of <jats:italic>Zi Shen Wan<\/jats:italic>, it demonstrated that ultra high performance liquid chromatography combined with quadrupole time\u2010of\u2010flight mass spectrometry coupled to MassLynx software and multivariate data processing approach could be successfully applied for rapid screening and comprehensive analysis of chemical constituents in vitro and prototype components or metabolites in vivo of traditional Chinese medicine.<\/jats:p>","DOI":"10.1002\/jssc.201600812","type":"journal-article","created":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T00:56:50Z","timestamp":1477357010000},"page":"4700-4711","source":"Crossref","is-referenced-by-count":36,"title":["Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of <i>Zi Shen Wan<\/i> using high\u2010throughput UHPLC\u2013MS with a multivariate analysis approach"],"prefix":"10.1002","volume":"39","author":[{"given":"Xian\u2010Na","family":"Li","sequence":"first","affiliation":[{"name":"Sino\u2010America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sino\u2010America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics Heilongjiang University of Chinese Medicine  Harbin 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Zou","sequence":"additional","affiliation":[{"name":"Sino\u2010America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics Heilongjiang University of Chinese Medicine  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xijun","family":"Wang","sequence":"additional","affiliation":[{"name":"Sino\u2010America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics Heilongjiang University of Chinese Medicine  Harbin 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