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A polymerization mixture containing 4\u2010vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine <jats:italic>cis\u2010diol<\/jats:italic> functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg\/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow\u2010rate and a flow\u2010rate of flushing buffer were selected as the most significant variables. 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