{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:13:34Z","timestamp":1762431214716,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2016,10,31]],"date-time":"2016-10-31T00:00:00Z","timestamp":1477872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006588","name":"Ministarstvo Znanosti, Obrazovanja i Sporta","doi-asserted-by":"publisher","award":["119\u20101191192\u20101222","098\u20100982913\u20102850"],"award-info":[{"award-number":["119\u20101191192\u20101222","098\u20100982913\u20102850"]}],"id":[{"id":"10.13039\/501100006588","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008993","name":"Sveu\u010dili\u0161te u Zagrebu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004963","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["316289"],"award-info":[{"award-number":["316289"]}],"id":[{"id":"10.13039\/501100004963","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>The serotype specificity of adenovirus ion\u2010exchange chromatography has previously been studied using standard particle\u2010based columns, and the hexon protein has been reported to determine retention time. In this study, we have submitted <jats:italic>Adenovirus type 5<\/jats:italic> recombinants to anion\u2010exchange chromatography using methacrylate monolithic supports. Our experiments with hexon\u2010modified adenoviral vectors show more precisely that the retention time is affected by the substitution of amino acids in hypervariable region 5, which lies within the hexon DE1 loop. By exploring the recombinants modified in the fiber protein, we have proven the previously predicted chromatographic potential of this surface constituent. Modifications that preserve the net charge of the hexon protein, or those that cause only a small charge difference in the fiber protein, in addition to shortening the fiber shaft, did not change the chromatographic behavior of the adenovirus particles. However, modifications that include the deletion of just two negatively charged amino acids in the hexon protein, or the introduction of a heterologous fiber protein, derived from another serotype, revealed recognizable changes in anion\u2010exchange chromatography. This could be useful in facilitating chromatography\u2010approach purification by creating targeted capsid modifications, thereby shifting adenovirus particles away from particular interfering substances present in the crude lysate.<\/jats:p>","DOI":"10.1002\/jssc.201600829","type":"journal-article","created":{"date-parts":[[2016,9,23]],"date-time":"2016-09-23T08:56:40Z","timestamp":1474621000000},"page":"4299-4304","source":"Crossref","is-referenced-by-count":5,"title":["Manipulating adenoviral vector ion\u2010exchange chromatography: Hexon versus fiber"],"prefix":"10.1002","volume":"39","author":[{"given":"Jelena","family":"Ru\u0161\u010di\u0107","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreja","family":"Ambriovi\u0107\u2010Ristov","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Laboratory for Cell Biology and Signaling Ru\u0111er Bo\u0161kovi\u0107 Institute  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dragomira","family":"Majhen","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Laboratory for Cell Biology and Signaling Ru\u0111er Bo\u0161kovi\u0107 Institute  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Kolund\u017eija","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milo\u0161","family":"Barut","sequence":"additional","affiliation":[{"name":"BIA Separations  Ajdov\u0161\u010dina Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Benihoud","sequence":"additional","affiliation":[{"name":"Univ Paris\u2010Sud  Orsay Cedex France"},{"name":"CNRS UMR 8203, Vectorologie et th\u00e9rapeutiques anticanc\u00e9reuses Gustave Roussy  Villejuif Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mladen","family":"Kraja\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2013.235"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/v2102290"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2014.007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.11.081"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.09.019"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.01.097"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.02.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.11.010"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301190"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.1346"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301845"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.10.004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00238-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.6.5156-5161.1999"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.132"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00597-1"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201600829","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201600829","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201600829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:26:09Z","timestamp":1760880369000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201600829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,31]]},"references-count":16,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/jssc.201600829"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201600829","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2016,10,31]]}}}