{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T20:42:52Z","timestamp":1778100172051,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T00:00:00Z","timestamp":1501632000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81102884"],"award-info":[{"award-number":["81102884"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373888"],"award-info":[{"award-number":["81373888"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p>The root of <jats:italic>Cynanchum auriculatum<\/jats:italic> (<jats:italic>C. auriculatum<\/jats:italic>) Royle ex Wight has been shown to possess various pharmacological effects and has recently attracted much attention with respect to its potential role in antitumor activity. The C\u201021 steroidal glycosides are commonly accepted as the major active ingredients of <jats:italic>C. auriculatum<\/jats:italic>. In this study, the antitumor abilities of different extracted fractions of the root bark and the root tuber of <jats:italic>C. auriculatum<\/jats:italic> were investigated by using a 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assay in human cancer cell lines HepG2 and SMMC\u20107721. The results showed that the\u00a0chloroform\u00a0and ethyl\u00a0acetate fractions of the root tuber suppressed tumor cell growth strongly. To identify and characterize the chemical constituents of different active fractions, an ultra high performance liquid chromatography with triple\u2010quadrupole tandem mass spectrometry method was developed for\u00a0the simultaneous quantitation of eight C\u201021 steroidal glycosides. The analysis revealed that the C\u201021 steroidal glycosides were concentrated in the\u00a0chloroform\u00a0and ethyl\u00a0acetate fractions, and the total contents of different fractions in the root tuber were significantly higher than those of corresponding ones in the root bark. Furthermore, the C\u201021 steroidal glycosides based on different types of aglucones were prone in different medicinal parts of <jats:italic>C. auriculatum<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jssc.201601415","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T10:25:31Z","timestamp":1501669531000},"page":"3054-3063","source":"Crossref","is-referenced-by-count":13,"title":["Antitumor evaluation and multiple analysis on different extracted fractions of the root of <i>Cynanchum auriculatum<\/i> Royle ex Wight"],"prefix":"10.1002","volume":"40","author":[{"given":"Xin\u2010jie","family":"Wang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"State Key Laboratory of Natural Medicines Research Department of Pharmacognosy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010lin","family":"Li","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm  Jiangsu Province Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu\u2010hui","family":"Lv","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm  Jiangsu Province Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010yan","family":"Zuo","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"State Key Laboratory of Natural Medicines Research Department of Pharmacognosy China Pharmaceutical University  Nanjing P. R. China"},{"name":"Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm  Jiangsu Province Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010min","family":"Zhao","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm  Jiangsu Province Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010fang","family":"Ding","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"She\u2010ban","family":"Pu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines Research Department of Pharmacognosy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010hui","family":"Qian","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm  Jiangsu Province Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9549-8926","authenticated-orcid":false,"given":"Yun\u2010ru","family":"Peng","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"13","article-title":"The current situations and progress of Baishouwu planted in Jiangsu","volume":"24","author":"Wang D. Y.","year":"2005","journal-title":"Chin. 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