{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T13:57:18Z","timestamp":1778680638796,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T00:00:00Z","timestamp":1493856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY15C200004"],"award-info":[{"award-number":["LY15C200004"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>The unicellular alga <jats:italic>Chlorella vulgaris<\/jats:italic> is a well\u2010known health food. It has been proven that the minor phytosterols, ergosterol and its analogue, are an important class of bioactive substances in <jats:italic>C. vulgaris<\/jats:italic>. In this work, a recycling counter\u2010current chromatographic approach was proposed for preparative separation of two analogue sterols from crude extract of <jats:italic>C. vulgaris<\/jats:italic>. The separation unit was set up with a type\u2010<jats:italic>J<\/jats:italic> instrument coupled with a column switching valve. A two\u2010phase solvent system composed of <jats:italic>n<\/jats:italic>\u2010hexane\/dichloromethane\/acetonitrile (10:3:7, v\/v\/v) was selected and optimized. After five cycles of separation, two analogue sterols were baseline separated, producing 11.7 mg 26\u2010nor\u201025\u2010isopropyl\u20105,7,22\u2010trien\u20103\u03b2\u2010ol and 20.3 mg ergosterol from 300 mg of <jats:italic>C. vulgaris<\/jats:italic> extract. Their purities were both above 95%. The structures of two sterols were identified by using NMR spectroscopy.<\/jats:p>","DOI":"10.1002\/jssc.201601434","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T13:05:58Z","timestamp":1490965558000},"page":"2326-2334","source":"Crossref","is-referenced-by-count":11,"title":["Preparative separation of phytosterol analogues from green alga <i>Chlorella vulgaris<\/i> using recycling counter\u2010current chromatography"],"prefix":"10.1002","volume":"40","author":[{"given":"Jianbing","family":"Wu","sequence":"first","affiliation":[{"name":"The Criminal Science and Technology Department Zhejiang Police College  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunping","family":"Liu","sequence":"additional","affiliation":[{"name":"Key laboratory of aquatic products processing of Zhejiang Province Institute of Seafood Zhejiang Gongshang University  Hangzhou P. R. 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