{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:27:59Z","timestamp":1770964079386,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T00:00:00Z","timestamp":1496966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["BS2015YY035, ZR2016YL006"],"award-info":[{"award-number":["BS2015YY035, ZR2016YL006"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008118","name":"Shandong Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008118","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>Five terpenoids, including two new ones, 3,7\u2010dioxo\u2010tirucalla\u20108,24\u2010dien\u201021\u2010oic acid (<jats:bold>2<\/jats:bold>) and 3\u03b1\u2010acetoxyl\u20107\u2010oxo\u2010tirucalla\u20108,24\u2010dien\u201021\u2010oic acid (<jats:bold>3<\/jats:bold>), and three known ones, boscartol A (<jats:bold>1<\/jats:bold>), 11\u2010keto\u2010\u03b2\u2010boswellic acid (<jats:bold>4<\/jats:bold>), and acetyl\u201011\u2010keto\u2010boswellic acid (<jats:bold>5<\/jats:bold>), have been extracted by the ultrapressure extraction and purified by pH\u2010zone\u2010refining countercurrent chromatography and high\u2010speed countercurrent chromatography from olibanum. For ultrapressure extraction, the optimal condition including 200 MPa of extraction pressure, ethyl acetate of extraction solvent, 1:20 (g\/mL) of solid\/liquid ratio, and 2\u00a0min of extraction time were obtained. For the separation, from 1.5 g of the terpenoid extract, 220.1\u00a0mg of <jats:bold>4<\/jats:bold>, 255.5\u00a0mg of <jats:bold>5<\/jats:bold>, and 212.3\u00a0mg of the mixture of <jats:bold>1<\/jats:bold>, <jats:bold>2<\/jats:bold>, and <jats:bold>3<\/jats:bold> were obtained by pH\u2010zone\u2010refining countercurrent chromatography under the solvent system of chloroform\/ethyl acetate\/methanol\/water (3:1:3:2, v\/v\/v\/v) with aqueous ammonia and trifluoroacetic acid as retention and eluter agents. The enriched mixture (210\u00a0mg) was further separated by conventional high\u2010speed countercurrent chromatography with petroleum ether\/ethyl acetate\/methanol\/water (1:0.8:1.1:0.6, v\/v\/v\/v), yielding 30.1\u00a0mg of <jats:bold>1<\/jats:bold>, 35.5\u00a0mg of <jats:bold>2<\/jats:bold>, 12.3\u00a0mg of <jats:bold>3<\/jats:bold>. The structures of these five terpenoids were elucidated by extensive spectroscopic methods.<\/jats:p>","DOI":"10.1002\/jssc.201700215","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T19:52:59Z","timestamp":1495569179000},"page":"2732-2740","source":"Crossref","is-referenced-by-count":25,"title":["Extraction and purification of five terpenoids from olibanum by ultrahigh pressure technique and high\u2010speed countercurrent chromatography"],"prefix":"10.1002","volume":"40","author":[{"given":"Jinqian","family":"Yu","sequence":"first","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology Shandong Analysis and Test Center  Jinan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology Shandong Analysis and Test Center  Jinan P.R. 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