{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:12:53Z","timestamp":1760879573605,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grand Project of Science Research for the 12th Five-year Plan Funded by Ministry of Science & Technology of China","award":["2012ZX09102-101-016"],"award-info":[{"award-number":["2012ZX09102-101-016"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities of China","doi-asserted-by":"crossref","award":["2016YXMS141"],"award-info":[{"award-number":["2016YXMS141"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enantioselective biodistribution studies of 1\u2010[4\u2010(2\u2010methoxyethyl)phenoxy]\u20103\u2010[2\u2010(2\u2010methoxyphenoxy)ethylamino]\u20102\u2010propanol hydrochloride (TJ0711), a novel antihypertensive agent, require the accurate and precise quantification of each TJ0711 enantiomer in biological fluids and tissues. Here we report a simple and sensitive liquid chromatography with tandem mass spectrometry method for simultaneous determination of (<jats:italic>R<\/jats:italic>)\u2010TJ0711 and (<jats:italic>S<\/jats:italic>)\u2010TJ0711 in rat plasma and tissue samples using protein precipitation. The influence of column type, temperature, mobile phase composition, and flow rate on the retention and enantioselectivity was evaluated. The separation of the TJ0711 enantiomers was ultimately achieved on a SUMICHIRAL OA\u20102500 column in 15\u00a0min using isocratic elution with ethanol\/hexane (40:60) at a flow rate of 0.8\u00a0mL\/min. Good linearities of spiked analyte concentration from 5 to 2000\u00a0ng\/mL were achieved and the correlation coefficients (<jats:italic>R<\/jats:italic>) were greater than 0.99. The intra\u2010 and inter\u2010day accuracy and precision for both analytes were &lt;15% at all concentration levels, and the extraction recoveries were consistent among the five quality control concentrations. This assay was successfully applied to quantify plasma and tissue concentrations of TJ0711 enantiomers in a preclinical study.<\/jats:p>","DOI":"10.1002\/jssc.201700710","type":"journal-article","created":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T02:52:45Z","timestamp":1503888765000},"page":"4135-4141","source":"Crossref","is-referenced-by-count":3,"title":["Enantioselective determination of 1\u2010[4\u2010(2\u2010methoxyethyl)phenoxy]\u20103\u2010[2\u2010(2\u2010methoxyphenoxy)ethylamino]\u20102\u2010propanol hydrochloride, a novel antihypertensive agent, in rat plasma and tissues by liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Sijie","family":"He","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanlan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Union Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeli","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luqin","family":"Si","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9298-2467","authenticated-orcid":false,"given":"Jiangeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Tongji Medical College Huazhong University of Science and Technology  Wuhan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2004.01.020"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","unstructured":"Lindholm L. H. Carlberg B. Samuelsson O. Should beta blockers remain first choice in the treatment of primary hypertension? 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