{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:24:37Z","timestamp":1760919877511,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T00:00:00Z","timestamp":1517875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Education of Zhejiang Province of China","award":["pd2013031"],"award-info":[{"award-number":["pd2013031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This work deals with the enantioseparation of \u03b1\u2010amino acids by chiral ligand exchange high\u2010speed countercurrent chromatography using <jats:italic>N<\/jats:italic>\u2010<jats:italic>n<\/jats:italic>\u2010dodecyl\u2010<jats:sc>l<\/jats:sc>\u2010hydroxyproline as a chiral ligand and copper(II) as a transition metal ion. A biphasic solvent system composed of <jats:italic>n<\/jats:italic>\u2010hexane\/<jats:italic>n<\/jats:italic>\u2010butanol\/aqueous phase with different volume ratios was selected for each \u03b1\u2010amino acid. The enantioseparation conditions were optimized by enantioselective liquid\u2013liquid extractions, in which the main influence factors, including type of chiral ligand, concentration of chiral ligand and transition metal ion, separation temperature, and pH of the aqueous phase, were investigated for racemic phenylalanine. Altogether, we tried to enantioseparate 15 racemic \u03b1\u2010amino acids by the analytical countercurrent chromatography, of which only five of them could be successfully enantioseparated. Different elution sequence for phenylalanine enantiomer was observed compared with traditional liquid chromatography and the proposed interactions between chiral ligand, transition metal ion (Cu<jats:sup>2+<\/jats:sup>), and enantiomer are discussed.<\/jats:p>","DOI":"10.1002\/jssc.201701117","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T11:38:54Z","timestamp":1515670734000},"page":"1479-1488","source":"Crossref","is-referenced-by-count":9,"title":["Chiral ligand exchange countercurrent chromatography: Enantioseparation of amino acids"],"prefix":"10.1002","volume":"41","author":[{"given":"Qing","family":"Xiong","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Biology Engineering Taizhou Vocational College of Science &amp; Technology  Taizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqiong","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhisi","family":"Bu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5867-7354","authenticated-orcid":false,"given":"Shengqiang","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2016.01.016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.08.041"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.01.020"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3016"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-015-2933-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.36662"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)95566-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)80308-8"},{"key":"e_1_2_6_10_1","first-page":"207","volume-title":"Methods in Molecular Biology","author":"Davankov V. 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