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The first\u2010dimensional countercurrent separation using a methylene chloride\/methanol\/water (11:6:5, v\/v\/v) system yielded three subfractions (fractions I\u2013III). The second\u2010dimensional countercurrent separations, conducted on fractions I\u2013III using <jats:italic>n<\/jats:italic>\u2010hexane<jats:italic>\/<\/jats:italic>ethyl acetate\/methanol\/water\/acetic acid (5:5:5:5:0, 3:7:3:7:0, and 1:9:1:9:0.01, v\/v\/v\/v\/v) systems, gave maltol (<jats:bold>1<\/jats:bold>), (\u2212)\u2010(13<jats:italic>S<\/jats:italic>)\u2010cuscutamine (<jats:bold>2<\/jats:bold>), (+)\u2010(13<jats:italic>R<\/jats:italic>)\u2010cuscutamine (<jats:bold>3<\/jats:bold>), (+)\u2010pinoresinol (<jats:bold>4<\/jats:bold>), (+)\u2010epipinoresinol (<jats:bold>5<\/jats:bold>), kaempferol (<jats:bold>6<\/jats:bold>), piperitol (<jats:bold>7<\/jats:bold>), and (9<jats:italic>R<\/jats:italic>)\u2010hydroxy\u2010<jats:sc>d<\/jats:sc>\u2010sesamin (<jats:bold>8<\/jats:bold>). To the best of our knowledge, maltol was identified for the first time in <jats:italic>Cuscuta<\/jats:italic> species. Furthermore, this report details the first full assignment of spectroscopic data of two cuscutamine epimers, (\u2212)\u2010(13<jats:italic>S<\/jats:italic>)\u2010cuscutamine and (+)\u2010(13<jats:italic>R<\/jats:italic>)\u2010cuscutamine.<\/jats:p>","DOI":"10.1002\/jssc.201701498","type":"journal-article","created":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T00:52:40Z","timestamp":1518742360000},"page":"2169-2177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Application of off\u2010line two\u2010dimensional high\u2010performance countercurrent chromatography on the chloroform\u2010soluble extract of <i>Cuscuta auralis<\/i> seeds"],"prefix":"10.1002","volume":"41","author":[{"given":"Taewoong","family":"Rho","sequence":"first","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9124-6935","authenticated-orcid":false,"given":"Kee Dong","family":"Yoon","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.09.032"},{"key":"e_1_2_7_3_1","first-page":"1326","article-title":"Effects of a polysaccharide from CCL on inhibiting oxygen free radical threshold of senile mice model","volume":"27","author":"Cai X. 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