{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T01:47:45Z","timestamp":1770515265711,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T00:00:00Z","timestamp":1520812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education, Youth and Sports of the Czech Republic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>New psychoactive substances represent serious social and health problem as tens of new compounds are detected in Europe annually. They often show structural proximity or even isomerism, which complicates their analysis. Two methods based on ultra high performance supercritical fluid chromatography and ultra high performance liquid chromatography with mass spectrometric detection were validated and compared. A simple dilute\u2010filter\u2010and\u2010shoot protocol utilizing propan\u20102\u2010ol or methanol for supercritical fluid or liquid chromatography, respectively, was proposed to detect and quantify 15 cathinones and phenethylamines in human urine. Both methods offered fast separation (&lt;3\u00a0min) and short total analysis time. Precision was well &lt;15% with a few exceptions in liquid chromatography. Limits of detection in urine ranged from 0.01 to 2.3\u00a0ng\/mL, except for cathinone (5\u00a0ng\/mL) in supercritical fluid chromatography. Nevertheless, this technique distinguished all analytes including four pairs of isomers, while liquid chromatography was unable to resolve fluoromethcathinone regioisomers. Concerning matrix effects and recoveries, supercritical fluid chromatography produced more uniform results for different compounds and at different concentration levels. This work demonstrates the performance and reliability of supercritical fluid chromatography and corroborates its applicability as an alternative tool for analysis of new psychoactive substances in biological matrixes.<\/jats:p>","DOI":"10.1002\/jssc.201800006","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T14:43:39Z","timestamp":1519224219000},"page":"2288-2295","source":"Crossref","is-referenced-by-count":20,"title":["Analysis of new psychoactive substances in human urine by ultra\u2010high performance supercritical fluid and liquid chromatography: Validation and comparison"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1470-3933","authenticated-orcid":false,"given":"Lucie","family":"Borovcov\u00e1","sequence":"first","affiliation":[{"name":"Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science Palack\u00fd University in Olomouc  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1313-2032","authenticated-orcid":false,"given":"Volodymyr","family":"Pauk","sequence":"additional","affiliation":[{"name":"Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science Palack\u00fd University in Olomouc  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3158-0637","authenticated-orcid":false,"given":"Karel","family":"Lemr","sequence":"additional","affiliation":[{"name":"Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science Palack\u00fd University in Olomouc  Olomouc Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,12]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"European Monitoring Centre for Drugs and Drug Addiction.European Drug Report 2017: Trends and Developments. 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