{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T12:36:20Z","timestamp":1773232580862,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T00:00:00Z","timestamp":1530576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Program of Shanghai Academic\/ Technology Research","award":["17XD1403500"],"award-info":[{"award-number":["17XD1403500"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Picrasma quassioides<\/jats:italic> (D. Don) Benn. is a widely used traditional Chinese medicine for anti\u2010inflammation and antibiosis. Canthinone and \u03b2\u2010carboline alkaloids are the main characteristic constituents that possess diverse pharmacological effects, such as anti\u2010inflammatory and anti\u2010infectious properties. In this study, bioautography in thin\u2010layer chromatography indicated that the antiradical activity compound may be alkaloids. Then, a simple, fast, and efficient method was established for the separation and purification of two types of alkaloids from <jats:italic>P. quassioides<\/jats:italic> by mass\u2010spectrometry\u2010directed autopurification system. Eight alkaloids were isolated and purified in this one\u2010step methodology. Among them, five compounds (<jats:bold>3<\/jats:bold>, 95.1%, 58.8\u00a0mg; <jats:bold>4<\/jats:bold>, 98.4%, 71.7\u00a0mg; <jats:bold>6<\/jats:bold>, 97.8%, 365.4\u00a0mg; <jats:bold>7<\/jats:bold>, 97.7%, 172.7\u00a0mg; <jats:bold>8<\/jats:bold>, 98.2%, 180.3\u00a0mg) were obtained in large amounts with extremely high purities. Then, the antiradical activities of the isolates showed that 4\u2010methoxy\u20105\u2010hydroxycanthin\u20106\u2010one (<jats:bold>6<\/jats:bold>) exhibited obvious 1,1\u2010diphenyl\u20102\u2010picryl\u2010hydrazyl free radical scavenging activity with an IC<jats:sub>50<\/jats:sub> value of 84.037 \u03bcM. This study offers a new method for the preparation of targeted bioactive alkaloids in <jats:italic>P. quassioides<\/jats:italic>. This work also provides a reference for the separation of other targeted chemical components with potential activities from traditional Chinese herbal medicines.<\/jats:p>","DOI":"10.1002\/jssc.201800127","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T08:24:42Z","timestamp":1527150282000},"page":"3014-3021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Preparation and purification of canthinone and \u03b2\u2010carboline alkaloids from <i>Picrasma quassioides<\/i> based on bioautography and mass\u2010spectrometry\u2010directed autopurification system"],"prefix":"10.1002","volume":"41","author":[{"given":"Na","family":"Yang","sequence":"first","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Center for Chinese Medical Therapy and Systems Biology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Shi","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aizhen","family":"Xiong","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Gu","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Center for Chinese Medical Therapy and Systems Biology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengtao","family":"Wang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,3]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"200","volume-title":"Pharmacopoeia of the People's Republic of China","author":"China Chinese Pharmacopoeia Committee Commission","year":"2015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200025"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.2996"},{"key":"e_1_2_9_5_1","first-page":"1149","article-title":"Chemical and pharmacological research progress of Picrasma quassioides","volume":"32","author":"Zhao W. 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