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Six tetracyclines were separated through a simple and effective isocratic procedure using a mixture of acetonitrile and 3.5\u00a0mM methanesulfonic acid. After that, intensive chemiluminescence signals were produced by the reaction between cerium(IV) and methoxylated cypridina luciferin analogues system in the presence of tetracyclines. Under the appropriate experimental conditions, the developed method was evaluated by testing of linearity, limits of detection, precision, and accuracy. The limit of detection was from 2 to 10\u00a0nM and relative standard deviation of intraday precision was below 9%. The developed high\u2010performance liquid chromatography with chemiluminescence method has been performed for the with trace analysis of tetracycline antibiotic residues in milk samples with satisfactory results.<\/jats:p>","DOI":"10.1002\/jssc.201800683","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T09:48:21Z","timestamp":1536832101000},"page":"4115-4121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Selective and sensitive determination of tetracyclines by HPLC with chemiluminescence detection based on a cerium(IV)\u2010methoxylated cypridina luciferin analogue system"],"prefix":"10.1002","volume":"41","author":[{"given":"Jie","family":"Li","sequence":"first","affiliation":[{"name":"Jiangxi Province Key Laboratory of Preventive Medicine School of Public Health Nanchang University  Nanchang P. R. 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