{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T13:25:46Z","timestamp":1781184346811,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7132031"],"award-info":[{"award-number":["7132031"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Yougui pills are a classic Chinese medicine that shows significant effects on nerve regeneration and neuroprotection in modern pharmacological studies. With a complex formula, Yougui pills have faced significant challenges in the fields of bioanalysis and pharmacokinetics in animals and human studies. In the present study, a specific and accurate high\u2010performance liquid chromatography with tandem mass spectrometry method was developed and validated for the quantitative determination of the six bioactive components in rat plasma after oral administration of Yougui pills. Chromatographic separation was performed on a C18 column with a gradient elution system. Samples were analysed using positive ion mode with multiple reaction monitoring mode. The assay showed good linearity for all six bioactive components in the dynamic range of 0.50 to 50\u00a0ng\/mL with acceptable intra\u2010 and inter\u2010batch accuracy and precision. The lower limits of quantification were 0.50\u00a0ng\/mL for all six bioactive components. The method was successfully applied to rat pharmacokinetics after oral administration of Yougui pills. All six bioactive components were detected in rat plasma, including songorine, benzoylhypaconitine, benzoylmesaconitine, neoline, karacoline, and sweroside, while some other target compounds were not detected, such as rhmannioside A, loganin, and cornuside I. After oral administration of Yougui pills at a dose of 2500\u00a0mg\/kg, all six bioactive components were rapidly absorbed, resulting in <jats:italic>t<\/jats:italic><jats:sub>max<\/jats:sub> values less than 1\u00a0h and relative lower <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> values. The <jats:italic>t<\/jats:italic><jats:sub>1\/2<\/jats:sub> values for songorine, benzoylhypaconitine, benzoylmesaconitine, neoline, karacoline, and sweroside were calculated to be 2.62\u00a0\u00b1\u00a00.67, 2.11\u00a0\u00b1\u00a00.45, 1.94\u00a0\u00b1\u00a00.35, 1.88\u00a0\u00b1\u00a00.31, 2.07\u00a0\u00b1\u00a00.44, and 1.59\u00a0\u00b1\u00a00.30\u00a0h, which indicated that Yougui pills should be taken in multiple oral doses over a relatively short period.<\/jats:p>","DOI":"10.1002\/jssc.201800772","type":"journal-article","created":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T03:57:00Z","timestamp":1552535820000},"page":"1867-1877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous high\u2010performance liquid chromatography with tandem mass spectrometry quantification of six bioactive components in rat plasma after oral administration of Yougui pill"],"prefix":"10.1002","volume":"42","author":[{"given":"Haolong","family":"Liu","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing Key Lab of TCM Collateral Disease Theory Research Capital Medical University  Beijing P. 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