{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:42:04Z","timestamp":1790019724898,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T00:00:00Z","timestamp":1549324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Marinobufagenin is a bufadienolide compound detected mainly in skin and parotoid gland secretions of <jats:italic>Rhinella marina<\/jats:italic> (L.) toad. Bufadienolides regulate the Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase pump by inhibiting the cardiotonic steroid dependent\u2010site and act as cardiac inotropes with vasoconstrictive properties. Marinobufagenin and other bufadienolides, such as telocinobufagin and bufalin, are thought to be found endogenously in mammals in salt\u2010sensitive hypertensive states such as essential hypertension, congestive heart\u2010failure, and preeclampsia. The role of marinobufagenin as antimicrobial agent and its cytotoxic potential have also been recognized. The particular interest around marinobufagenin prompts us to consider the <jats:italic>Rhinella marina<\/jats:italic> toad venom as a possible source for molecules with pharmacological and\/or diagnostic potential. In this article, two different approaches of extraction and purification of marinobufagenin from <jats:italic>Rhinella marina<\/jats:italic> (L.) venom are studied: (i) Preparative thin\u2010layer chromatography combined to mass spectrometry and\/or ultraviolet detection and (ii) solid\u2010phase extraction coupled with fractionation on high\u2010performance liquid chromatography. Different chromatographic conditions are tested for each approach. The solid\u2010phase extraction combined with high\u2010performance liquid chromatography fractionation approach was preferred as it offered a greater yield, was less time\u2010consuming and allowed us to selectively isolate marinobufagenin. Both protocols aim to provide efficient and convenient methods for toad venom extraction, based on an easily automatable and systematized strategy.<\/jats:p>","DOI":"10.1002\/jssc.201800879","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T16:45:34Z","timestamp":1548261934000},"page":"1384-1392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Marinobufagenin extraction from <i>Rhinella marina<\/i> toad glands: Alternative approaches for a systematized strategy"],"prefix":"10.1002","volume":"42","author":[{"given":"Charline","family":"Lenaerts","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Faculty of medicine and pharmacy Research Institute for Health Sciences and Technology University of Mons  Mons Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Wells","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Faculty of medicine and pharmacy Research Institute for Health Sciences and Technology University of Mons  Mons Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Hamb\u00ffe","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Faculty of medicine and pharmacy Research Institute for Health Sciences and Technology University of Mons  Mons Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6667-7677","authenticated-orcid":false,"given":"Bertrand","family":"Blankert","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Faculty of medicine and pharmacy Research Institute for Health Sciences and Technology University of Mons  Mons Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(97)00426-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0001-37652005000100004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2017.01.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.02.028"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.09.026"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1997.tb00018.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(87)90265-0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","unstructured":"Porto A. M. Baralle F. E. Gros E. G. Biosynthesis of bufadienolides in toads. III \u2010 Experiments with mevalonic acid hydroxy pregnen ona and cholesterol.1972 3.","DOI":"10.1016\/0022-4731(72)90006-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.12.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/a815397y"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","unstructured":"Cunha\u2010filho G. C W. H. P. Bosch R. A. Alonso I. P. Pereda\u2010miranda R. Gervou R. Eduardo L. Quintas M. No F. Toxicon Bufadienolides from parotoid gland secretions of Cuban toad Peltophryne fustiger (Bufonidae): Inhibition of human kidney Na \u00fe \/ K \u00fe \u2010ATPase activity. 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Quispe C. Vargas G. Toxicon Antiproliferative activity and chemical composition of the venom from the Amazonian toad Rhinella marina (Anura\u202f: Bufonidae). Toxicon 2016 121 119\u2013129.","DOI":"10.1016\/j.toxicon.2016.09.004"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.03.021"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)00735-P"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/01483919008051801"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","unstructured":"Cunha G. A. Alberto C. Lemos S. Castro M. S. Ma M. Leite R. S. Kyaw C. Pires O. R. Bloch C. Ferroni E. Antimicrobial activity of the bufadienolides marinobufagin and telocinobufagin isolated as major components from skin secretion of the toad Bufo rubescens. 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