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A solvent system composed of <jats:italic>n<\/jats:italic>\u2010hexane\/methanol\/formic acid aqueous solution with pH\u00a0=\u00a03 (10:6:4, v\/v) was screened to achieve satisfactory isolation from the ethanol extract of pigeon pea leaves. Four compounds, 9.2\u00a0mg of compound <jats:bold>1<\/jats:bold> (96.8%), 3.2\u00a0mg of <jats:bold>2<\/jats:bold> (88.0%), 6.2\u00a0mg of <jats:bold>4<\/jats:bold> (94.2%) and 25.2\u00a0mg of <jats:bold>5<\/jats:bold> (94.2%), were obtained by conventional elution from 100\u00a0mg of the precipitation fraction, respectively. Two compounds, 14.4\u00a0mg of <jats:bold>3<\/jats:bold> (96.3%) and 28.1\u00a0mg of <jats:bold>6<\/jats:bold> (96.6%), with high <jats:italic>K<\/jats:italic> values were obtained by the subsequent extrusion procedure. The compounds <jats:bold>1<\/jats:bold>\u2013<jats:bold>6<\/jats:bold> were identified as 3\u2010methoxy\u20105\u2010(2\u2010phenylethenyl)\u2010phenol, pinostrobin chalcone, pinostrobin, 2\u2010hydroxy\u20104\u2010methoxy\u20106\u2010(2\u2010phenylvinyl)\u2010benzoic acid, longistylin C and cajaninstilbene acid by quadrupole time\u2010of\u2010flight mass spectrometry, and <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR spectroscopy. The <jats:italic>in vitro<\/jats:italic> antiproliferation activities of compounds <jats:bold>1<\/jats:bold>, <jats:bold>5<\/jats:bold> and <jats:bold>6<\/jats:bold> against human hepatoma cell were evaluated and the half\u2010maximum inhibitory concentrations were acquired.<\/jats:p>","DOI":"10.1002\/jssc.201801111","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T11:50:04Z","timestamp":1547725804000},"page":"1202-1209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Separation of six compounds from pigeon pea leaves by elution\u2013extrusion counter\u2010current chromatography"],"prefix":"10.1002","volume":"42","author":[{"given":"Xiaoai","family":"He","sequence":"first","affiliation":[{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals College of Pharmaceutical Sciences Zhejiang University of Technology  Hangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huichen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals College of Pharmaceutical Sciences Zhejiang University of Technology  Hangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8633-3724","authenticated-orcid":false,"given":"Xianrui","family":"Liang","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals College of Pharmaceutical Sciences Zhejiang University of Technology  Hangzhou P. R. 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