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Using the stoichiometric interaction in molecular imprinting, no excess of binding sites is necessary and binding sites are only located inside the imprinted cavities. The free base of the template was obtained to facilitate the interaction with the monomers. Itaconic acid was selected as the functional monomer, and stoichiometric ratio of the interaction with the free base was investigated. The molecularly imprinted polymer preparation conditions such as cross\u2010linker, molar ratio, porogen were optimized as divinylbenzene, 1:2:20 and chloroform\/N,N\u2010dimethylformamide, respectively. Under the optimum conditions, a good imprinting effect and very high selectivity were achieved. A solid\u2010phase extraction method was developed using the molecularly imprinted polymers as a sorbent and extraction procedure was optimized. The solid\u2010phase extraction method showed a high extraction recovery for auramine O in its hydrochloride form and free form compared to its analogues. The results strongly indicated that stoichiometric imprinting is an efficient method for development of high selectivity molecularly imprinted polymers for auramine O.<\/jats:p>","DOI":"10.1002\/jssc.201801234","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T05:31:11Z","timestamp":1550035871000},"page":"1634-1643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Preparation of a stoichiometric molecularly imprinted polymer for auramine O and application in solid\u2010phase extraction"],"prefix":"10.1002","volume":"42","author":[{"given":"Parezhati","family":"Pataer","sequence":"first","affiliation":[{"name":"Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education &amp; Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering Xinjiang University  Urumqi P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5562-9365","authenticated-orcid":false,"given":"Turghun","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education &amp; Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering Xinjiang University  Urumqi P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunusjan","family":"Turahun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education &amp; Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering Xinjiang University  Urumqi P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education &amp; Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering Xinjiang University  Urumqi P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sailemayi","family":"Aihebaier","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education &amp; Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering Xinjiang University  Urumqi P. R. 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