{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T01:57:19Z","timestamp":1778551039828,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T00:00:00Z","timestamp":1548806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21676302"],"award-info":[{"award-number":["21676302"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21878339"],"award-info":[{"award-number":["21878339"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new strategy by converging ultrafiltration high\u2010performance liquid chromatography with ultraviolet and mass spectrometry and pH\u2010zone\u2010refining counter\u2010current chromatography was developed for the rapid screening and separation of potential acetylcholinesterase inhibitors from the crude alkaloidals extract of <jats:italic>Zanthoxylum nitidum<\/jats:italic>. An optimized two\u2010phase solvent system composed of chloroform\/methanol\/water (4:3:3, v\/v) was used in this study. And, in the optimal solvent system, 45\u00a0mM hydrochloric acid was added to the aqueous stationary phase as the retainer, while 5\u00a0mM triethylamine was added to the organic mobile phase as the eluter. As a result, with the purity of over 95%, five alkaloids including jatrorrhizine (<jats:bold>1<\/jats:bold>, 340\u00a0mg), columbamine (<jats:bold>2<\/jats:bold>, 112\u00a0mg), skimmianine (<jats:bold>3<\/jats:bold>, 154\u00a0mg), palmatine (<jats:bold>4<\/jats:bold>, 226\u00a0mg), and epiberberine (<jats:bold>5<\/jats:bold>, 132\u00a0mg) were successfully purified in one step from 3.0\u00a0g crude alkaloidals extract. And their structures were identified by ultraviolet, mass spectrometry, <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR spectroscopy. Notably, compounds <jats:bold>2<\/jats:bold>, <jats:bold>4<\/jats:bold> and <jats:bold>5<\/jats:bold> were firstly reported in <jats:italic>Z. nitidum<\/jats:italic>. In addition, acetylcholinesterase inhibitory activities of compounds <jats:bold>1\u20135<\/jats:bold> were evaluated, and compounds <jats:bold>3, 4<\/jats:bold> and <jats:bold>5<\/jats:bold> exhibited stronger acetylcholinesterase inhibitory activity (IC<jats:sub>50<\/jats:sub> values at 8.52\u00a0\u00b1\u00a00.64, 14.82\u00a0\u00b1\u00a01.21 and 3.12\u00a0\u00b1\u00a00.32\u00a0\u03bcg\/mL, respectively) than berberine (IC<jats:sub>50<\/jats:sub> value at 32.86\u00a0\u00b1\u00a02.14\u00a0\u03bcg\/mL, positive control). The results indicated that the proposed method is an efficient technique to rapidly screen acetylcholinesterase inhibitors from complex samples, and could be served as a large\u2010scale preparative technique for separating ionizable active compounds.<\/jats:p>","DOI":"10.1002\/jssc.201801238","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T05:25:23Z","timestamp":1547443523000},"page":"1194-1201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Large\u2010scale separation of acetylcholinesterase inhibitors from <i>Zanthoxylum nitidum<\/i> by pH\u2010zone\u2010refining counter\u2010current chromatography target\u2010guided by ultrafiltration high\u2010performance liquid chromatography with ultraviolet and mass spectrometry screening"],"prefix":"10.1002","volume":"42","author":[{"given":"Minzhuo","family":"Liu","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering Central South University  Changsha P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Central South University  Changsha P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Central South University  Changsha P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqian","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Central South University  Changsha P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8768-8965","authenticated-orcid":false,"given":"Xiaoqing","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Central South University  Changsha P. R. China"},{"name":"Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety Central South University  Changsha P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.32.1433"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(03)00111-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1166(200003)15:3<203::AID-GPS100>3.0.CO;2-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1254\/fpj.115.45"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2008.02.016"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbacli.2016.09.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijge.2015.10.010"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.01.061"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659-018-0169-7"},{"key":"e_1_2_8_11_1","article-title":"New alkylamide from the stems of Zanthoxylum nitidum. Nat. Prod","author":"Chakthong S.","year":"2018","journal-title":"Res"},{"key":"e_1_2_8_12_1","first-page":"497","article-title":"Anti\u2010hepatoma activity of nitidine chloride and its effect on topoisomerase","volume":"26","author":"Liu L. M.","year":"2010","journal-title":"Chin Pharmacol Bull"},{"key":"e_1_2_8_13_1","first-page":"103","article-title":"Studies on the anti\u2010bacterial activity of different polarities from the roots and stems of Zanthoxylum nitidum (Roxb.) DC","volume":"29","author":"Huang Y. L.","year":"2013","journal-title":"Pharmacol Clin Chin Mater Med."},{"key":"e_1_2_8_14_1","first-page":"60","article-title":"Studies on the anti\u2010inflammatory and analgesic activities of different polarities from the roots and stems of Zanthoxylum nitidum (Roxb.) DC","volume":"27","author":"Feng J.","year":"2011","journal-title":"Pharmacol Clin Chin Mater Med."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2014.1002090"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/10286020.2015.1049161"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1076\/phbi.39.7.18.5867"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.12.015"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601216"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700258"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700835"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800691"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800658"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.12.044"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701181"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.11.024"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.04.007"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.05.048"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.12.016"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300971"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.12.025"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201000857"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201801238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201801238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.201801238","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201801238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:45:34Z","timestamp":1760874334000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201801238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,30]]},"references-count":32,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/jssc.201801238"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201801238","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,30]]},"assertion":[{"value":"2018-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}