{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T19:02:48Z","timestamp":1773428568146,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T00:00:00Z","timestamp":1556064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ14H280003"],"award-info":[{"award-number":["LQ14H280003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dan\u2010Huang\u2010Qu\u2010Yu capsule, a Chinese herbal medicine compound preparation, is widely used for chronic pelvic inflammatory disease. In this study, a rapid, selective, and sensitive microwave\u2010assisted extraction ultra\u2010high\u2010performance liquid chromatography\u2010Q Exactive quadrupole\u2010orbitrap high\u2010resolution mass spectrometry method was developed for analyzing its chemical compositions. A total of 85 compounds, including 22 flavonoids, 8 terpenoids, 5 quinones, 5 phthaleolactone, 23 organic acids, and 22 other compounds were identified from Dan\u2010Huang\u2010Qu\u2010Yu capsule. Among them, 35 major compounds were unambiguously detected by comparing them with reference standards and selected as quality control markers, which were simultaneously determined in Dan\u2010Huang\u2010Qu\u2010Yu capsule. The established method was successfully validated and applied for simultaneous determination of 35 bioactive compounds in Dan\u2010Huang\u2010Qu\u2010Yu capsule from ten sample batches. The quantitative data of the analytes were analyzed by principal component analysis for quality assessment of Dan\u2010Huang\u2010Qu\u2010Yu capsule. Six compounds (e.g., astragaloside IV, salvianolic acid B, ellagic acid, chlorogenic acid, <jats:italic>N<\/jats:italic>\u2010butylidenephthalide, and luteolin) were screened out and regarded as chemical markers for quality control of Dan\u2010Huang\u2010Qu\u2010Yu capsule. The established method has been proved to be a novel and useful tool for rapid research of Dan\u2010Huang\u2010Qu\u2010Yu capsule. This research will provide reference for the scientific research of traditional Chinese medicines.<\/jats:p>","DOI":"10.1002\/jssc.201801246","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T10:39:04Z","timestamp":1554287944000},"page":"2069-2079","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comprehensive characterization and quantification of multiple components in Dan\u2010Huang\u2010Qu\u2010Yu capsule using a multivariate data processing approach based on microwave\u2010assisted extraction with UHPLC and Q Exactive quadrupole\u2010orbitrap high\u2010resolution mass spectrometry"],"prefix":"10.1002","volume":"42","author":[{"given":"Bo","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Tongde Hospital Zhejiang Province  Hangzhou Zhejiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Tongde Hospital Zhejiang Province  Hangzhou Zhejiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the 117th Hospital of PLA  Hangzhou Zhejiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1757-7063","authenticated-orcid":false,"given":"Shuyu","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Tongde Hospital Zhejiang Province  Hangzhou Zhejiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"880","article-title":"Clinical trial of gestrinone capsules combined with Danhuang Quyu capsules in the treatment of adenomyosis","volume":"33","author":"Zhang Y. Z.","year":"2017","journal-title":"Chin. J. Clin. Pharmacol"},{"key":"e_1_2_8_3_1","first-page":"3705","article-title":"The multiple constituents study of Danhuang Quyu Capsule based on UPLC\u2010Q\u2010Orbitrap HRMS","volume":"48","author":"Li Z. L.","year":"2017","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_4_1","first-page":"2362","article-title":"Clinical Research on Danhuang Quyu Capsule in the Treatment of Sequelea of Pelvic Inflammatory Disease for the Protection of Chinese Materia Medica","volume":"12","author":"Shang W.","year":"2017","journal-title":"World Chiese Medicine"},{"key":"e_1_2_8_5_1","first-page":"844","article-title":"Clinical study on Danhuang Quyu Capsule combined with clindamycin in treatment of chronic pelvic inflammation","volume":"32","author":"Chen Q. Z.","year":"2017","journal-title":"Drugs & Clinic"},{"key":"e_1_2_8_6_1","first-page":"1522","article-title":"Study on the Effect of Dan Huang Quyu Capsule and Kangfu Xiaoyan Suppositoryon Reliving Chronic Pain due to PID and the Partial Mechanism","volume":"10","author":"Wu Y. C.","year":"2015","journal-title":"World Chiese Medicine"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.09.031"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemolab.2005.06.018"},{"key":"e_1_2_8_9_1","first-page":"157","article-title":"Determination of Sodium Danshensu in Danhuang Quyu Capsule by HPLC","volume":"40","author":"Jie S. Q.","year":"2012","journal-title":"Guangzhou Chemical Industry"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-013-2608-2"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700608"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201801107"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2014.10.056"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.01.024"},{"key":"e_1_2_8_15_1","unstructured":"http:\/\/www.ich.org\/fileadmin\/PublicWebSite\/ICHProducts\/Guidelines\/Quality\/Q2 R1\/Step4\/Q2 R1 Guideline.pdf."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.09.018"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.09.006"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2016.03.021"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2014.11.002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.08.036"},{"key":"e_1_2_8_21_1","volume-title":"Chemometrics: A Textbook","author":"Masssart D. L.","year":"1998"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2551"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201801246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201801246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.201801246","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201801246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:40:11Z","timestamp":1760884811000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201801246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,24]]},"references-count":21,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/jssc.201801246"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201801246","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,24]]},"assertion":[{"value":"2018-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}