{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:55:08Z","timestamp":1768870508605,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81202493"],"award-info":[{"award-number":["81202493"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012590","name":"Opening Project of Shanghai Key Laboratory of New Drug Design","doi-asserted-by":"publisher","award":["17DZ2271000"],"award-info":[{"award-number":["17DZ2271000"]}],"id":[{"id":"10.13039\/501100012590","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We present an ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time\u2010of\u2010flight mass spectrometry method for the simultaneous quantification of ten purines (adenine, hypoxanthine, guanine, xanthine, deoxyadenosine, adenosine, inosine, guanosine, xanthosine, and uric acid) and creatinine in human urine. After chromatographic separation on an ACE Excel 2 AQ column, high abundant creatinine and uric acid and the other low abundant purines were sequentially detected by ultraviolet and quadrupole time\u2010of\u2010flight mass spectrometry within a single run. Method validations including specificity (improved by accurate mass measurement), linearity (correlation coefficients \u22650.9944), limit of quantification (0.002\u20139.756\u00a0\u00b5g\/mL), intra\u2010 and interday precision (relative standard deviations \u22649.1 and 14.0%, respectively), accuracy (relative errors \u226413.1%), extraction recovery (between 90.3 and 109.6%), matrix effect (between 85.3 and 110.5%), and stability (relative errors \u226414.3%) were fully evaluated. This approach was applied to characterize the disordered purine metabolism in acute and chronic gout as an example. Quantitative results (normalized by creatinine) showed that an overproduction of urinary purine precursors might be involved in the gout process. The developed method represents a useful tool to investigate the purine disturbances in gout and other relevant diseases.<\/jats:p>","DOI":"10.1002\/jssc.201900170","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T02:48:25Z","timestamp":1559184505000},"page":"2523-2533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous quantification of urinary purines and creatinine by ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time\u2010of\u2010flight mass spectrometry: Method development, validation, and application to gout study"],"prefix":"10.1002","volume":"42","author":[{"given":"Yuan","family":"Yuan","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Engineering East China University of Science and Technology  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Jiujiang First People's Hospital  Jiujiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Engineering East China University of Science and Technology  Shanghai P. R. China"},{"name":"Shanghai Key Laboratory of New Drug Design School of Pharmacy East China University of Science and Technology  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Jiujiang First People's Hospital  Jiujiang P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of New Drug Design School of Pharmacy East China University of Science and Technology  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4836-9881","authenticated-orcid":false,"given":"Ping","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Engineering East China University of Science and Technology  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS19820048"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60883-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI35111"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2009.03.020"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.04.022"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN02294G"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.50"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-007-0091-1"},{"key":"e_1_2_8_10_1","first-page":"245415","article-title":"LC\u2010MS\u2010MS measurements of urinary creatinine and the application of creatinine normalization technique on cotinine in smokers\u2019 24 hour urine. J. Anal","volume":"2012","author":"Hou H. W.","year":"2012","journal-title":"Methods Chem."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201500055"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.me.39.020188.002341"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900672"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2mb25227a"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6388-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.06.065"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.09.023"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.02.026"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0086"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900727"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.07.094"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.01.111"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1728"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.12.076"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.02.043"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800734"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.04.074"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1539"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.07.038"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.040261"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201900170","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.201900170","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201900170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:01:32Z","timestamp":1760875292000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201900170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,17]]},"references-count":29,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1002\/jssc.201900170"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201900170","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,17]]},"assertion":[{"value":"2019-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}