{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T19:04:38Z","timestamp":1784315078437,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Melodamide A, a phenolic amide from the leaves of <jats:italic>Melodorum fruticosum<\/jats:italic> Lour., has previously shown pronounced anti\u2010inflammatory activity. In order to rapidly isolate larger quantities for biological testing, a fast, one\u2010step isolation method by centrifugal partition chromatography was developed within this study. Fractionation of the dichloromethane extract was performed with a two\u2010phase solvent system consisting of <jats:italic>n<\/jats:italic>\u2010hexane, ethyl acetate, methanol, and water (3:7:5:5, v\/v), leading to the isolation of melodamide A with a purity of &gt;90% and a yield of 6.7\u00a0w% within 32\u00a0min. The developed method can also be used in dual mode for the enrichment of further constituents like flavonoids or chalcones. In order to support the centrifugal partition chromatography method development, additionally, a high\u2010performance liquid chromatography method was established and validated to determine quantities of melodamide A in plant material and crude extracts. Analysis of <jats:italic>M. fruticosum<\/jats:italic> leaves and a dichloromethane extract obtained from this plant material showed a total melodamide A content of 0.19 \u00b1 0.008 and 8.9 \u00b1 0.249\u00a0w%, respectively.<\/jats:p>","DOI":"10.1002\/jssc.201900392","type":"journal-article","created":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T11:13:31Z","timestamp":1565262811000},"page":"3165-3172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Melodamide A from <i>Melodorum fruticosum<\/i> \u2014 Quantification using HPLC and one\u2010step\u2010isolation by centrifugal partition chromatography"],"prefix":"10.1002","volume":"42","author":[{"given":"Nora S.","family":"Engels","sequence":"first","affiliation":[{"name":"Institute of Pharmacy\/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI) University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2718-4677","authenticated-orcid":false,"given":"Birgit","family":"Waltenberger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy\/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI) University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwaiger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy\/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI) University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loi","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy University of Medicine and Pharmacy at H\u1ed3 Ch\u00ed Minh City   H\u1ed3 Ch\u00ed Minh Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy University of Medicine and Pharmacy at H\u1ed3 Ch\u00ed Minh City   H\u1ed3 Ch\u00ed Minh Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Stuppner","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy\/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI) University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,29]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Dictionary of Common Plants","author":"V\u00f5 V. C.","year":"2003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.31989\/ffhd.v5i1.162","article-title":"Formulation and characterization of novel functional beverages with antioxidant and anti\u2010acetylcholinesterase activities","volume":"5","author":"Nanasombat S.","year":"2015","journal-title":"Funct. Foods Health Dis."},{"key":"e_1_2_8_4_1","first-page":"39","article-title":"Physico\u2010chemical and sensory properties of musts and wines from Melodorum fruticosum Lour","volume":"21","author":"Tatdao P.","year":"2014","journal-title":"Int. Food Res. J."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1201\/b16504"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.07.002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50074a023"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)87811-X"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(90)80142-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(91)85123-H"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(01)00215-1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1328131"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201800269"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/np800144q"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/np501065h"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.12.044"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.10.018"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601221"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800516"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601063"},{"key":"e_1_2_8_21_1","unstructured":"International Conference on Harmonization International Federation of Pharmaceutical Manufacturers and Associations (IFPMA) Geneva1996."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)91626-7"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201900392","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.201900392","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201900392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:05:43Z","timestamp":1760864743000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201900392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,29]]},"references-count":21,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/jssc.201900392"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201900392","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,29]]},"assertion":[{"value":"2019-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}