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Beauverolides act as cholesterol acyltransferase inhibitors in humans; thus, their mode of action has been the subject of pharmacological and clinical research. The cost\u2010effective analytical methods are needed for fast, routine laboratory analysis of beauverolides. We isolated beauverolides from the fungal strain <jats:italic>Isaria fumosorosea<\/jats:italic> PFR 97\u2010Apopka and opened the rings of the isolated beauverolides using a pyridine alkaline medium. We separated fractions of cyclic and linearized beauverolides by thin\u2010layer chromatography, and found the chloroform\u2013acetate (9:1, v\/v) and chloroform\u2013acetonitrile\u2013acetate (8:1:1, v\/v\/v) mobile phases, respectively, to be the most efficient. We examined all the fractions by liquid chromatography\u2013mass spectrometry using ion trap and Orbitrap high resolution mass spectrometry. For rapid screening of the contents of cyclic, and, particularly, linearized beauverolides, we developed a novel analytical method that consisted of using capillary electrophoresis coupled with contactless conductivity detection. Furthermore, we improved the separation of the peptides by applying capillary micellar electrokinetic chromatography with the <jats:italic>N<\/jats:italic>\u2010cyclohexyl\u20102\u2010aminoethanesulfonic acid:SDS:NaOH buffer, pH 9.8 as the background electrolyte. The described novel methods allow fast and cost\u2010effective separation of chemically related groups of beauverolides.<\/jats:p>","DOI":"10.1002\/jssc.201901084","type":"journal-article","created":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T00:51:30Z","timestamp":1576198290000},"page":"962-969","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid methods for the separation of natural mixtures of beauverolides, cholesterol acyltransferase inhibitors, isolated from the fungus <i>Isaria fumosorosea<\/i>"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1227-846X","authenticated-orcid":false,"given":"Daniela","family":"\u0160im\u010d\u00edkov\u00e1","sequence":"first","affiliation":[{"name":"Charles University Third Faculty of Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3489-9547","authenticated-orcid":false,"given":"Petr","family":"T\u016fma","sequence":"additional","affiliation":[{"name":"Charles University Third Faculty of Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Alexandr","family":"Jegorov","sequence":"additional","affiliation":[{"name":"Charles University Third Faculty of Medicine  Prague Czech Republic"},{"name":"Biology Centre Czech Academy of Sciences  \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"\u0160imek","sequence":"additional","affiliation":[{"name":"Biology Centre Czech Academy of Sciences  \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0703-951X","authenticated-orcid":false,"given":"Petr","family":"Heneberg","sequence":"additional","affiliation":[{"name":"Charles University Third Faculty of Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)90402-3"},{"key":"e_1_2_9_3_1","first-page":"113","article-title":"Potent anti\u2010calmodulin activity of cyclotetradepsipeptides isolated from Isaria fumosorosea using a newly designed biosensor","volume":"10","author":"Madariaga\u2010Mazon A.","year":"2015","journal-title":"Nat. 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Jegorov A. \u0160imek P. Beauveriolide I(beauverolide I) 98%. 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