{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:37:20Z","timestamp":1781527040726,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T00:00:00Z","timestamp":1579824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010568","name":"Secretaria Xeral de Investigaci\u00f3n e Desenvolvemento, Xunta de Galicia","doi-asserted-by":"publisher","award":["6RC2014\/2016"],"award-info":[{"award-number":["6RC2014\/2016"]}],"id":[{"id":"10.13039\/501100010568","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010568","name":"Secretaria Xeral de Investigaci\u00f3n e Desenvolvemento, Xunta de Galicia","doi-asserted-by":"publisher","award":["ED431C2018\/19"],"award-info":[{"award-number":["ED431C2018\/19"]}],"id":[{"id":"10.13039\/501100010568","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010568","name":"Secretaria Xeral de Investigaci\u00f3n e Desenvolvemento, Xunta de Galicia","doi-asserted-by":"publisher","award":["ED431E2018\/08"],"award-info":[{"award-number":["ED431E2018\/08"]}],"id":[{"id":"10.13039\/501100010568","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although aflatoxins contamination in feedstuff is a well\u2010known problem, and hence these residues are controlled in poultry products, there is scarce information regarding the presence of these toxic substances in aquaculture fish, facilities that use several feedstuff for fish breeding. A simple, rapid, and sensitive method has been therefore developed for aflatoxins (B1, B2, G1, and G2) assessment in aquaculture products by combining ultrasound probe\u2010assisted extraction and vortex\u2010assisted liquid\u2013liquid microextraction as a sample pretreatment, and high\u2010performance liquid chromatography\u2010tandem mass spectrometry as a separation\/detection system. Aflatoxins were extracted from fish flesh\/liver with a 60:40 acetonitrile\/aqueous phosphate buffer (pH 7.0) mixture before preconcentration and clean\u2010up by vortex\u2010assisted liquid\u2013liquid microextraction under the following optimized conditions: 5.0\u00a0mL of fish extract at pH 7.0 and NaCl at 0.5% (w\/v), 400 \u03bcL of chloroform as extracting solvent, and vortex shaking at 2000\u00a0rpm for 1\u00a0min. The proposed method is shown to be precise and accurate, and the limit of quantitations (from 0.20 to 1.10\u00a0\u03bcg kg<jats:sup>\u22121<\/jats:sup>) were lower than the value established by the European Commission Regulation for aflatoxins in foodstuff. Results have shown that fish flesh is free of aflatoxins, but aflatoxins B2 and G1 were quantified in fish liver.<\/jats:p>","DOI":"10.1002\/jssc.201901129","type":"journal-article","created":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T11:44:14Z","timestamp":1579088654000},"page":"1331-1338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<b>C<\/b>ombining ultrasound\u2010assisted extraction and vortex\u2010assisted liquid\u2013liquid microextraction for the sensitive assessment of aflatoxins in aquaculture fish species"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7761-0788","authenticated-orcid":false,"given":"G. D. Thilini Madurangika","family":"Jayasinghe","sequence":"first","affiliation":[{"name":"Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT) Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry Universidade de Santiago de Compostela  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-3166","authenticated-orcid":false,"given":"Raquel","family":"Dom\u00ednguez\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT) Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry Universidade de Santiago de Compostela  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5864-6144","authenticated-orcid":false,"given":"Pilar","family":"Bermejo\u2010Barrera","sequence":"additional","affiliation":[{"name":"Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT) Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry Universidade de Santiago de Compostela  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3512-6434","authenticated-orcid":false,"given":"Antonio","family":"Moreda\u2010Pi\u00f1eiro","sequence":"additional","affiliation":[{"name":"Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT) Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry Universidade de Santiago de Compostela  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569541003598553"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.11.010"},{"key":"e_1_2_8_4_1","unstructured":"ARC Working Group on the Evaluation of Carcinogenic Risks to Humans I.A.f.R.o.C.a.W.H.O.Some naturally occurring substances: Food items and constituents heterocyclic aromatic amines and mycotoxins. World Health Organization World Health Organization1993."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2857-y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(01)00526-9"},{"key":"e_1_2_8_7_1","first-page":"396","article-title":"Effect of aflatoxin\u2010contaminated feed on morbidity and residues in walleye fish","volume":"35","author":"Hussain M.","year":"1993","journal-title":"Vet. Hum. Toxicol."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.04.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf1047354"},{"key":"e_1_2_8_10_1","unstructured":"COMMISSION REGULATION (EC). No 1881\/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. 2006R1881\u2014EN\u201401.07.2010\u2014 004.001\u20141\/26."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2772-2"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2010.04.050"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sab.2008.10.042"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.11.088"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2011.04.014"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-013-7467-z"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.02.009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201401267"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201801107"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701282"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800359"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2009.11.005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701167"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800388"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800921"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201900457"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-017-3403-2"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.11.007"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2015.02.025"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.05.026"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900329"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.01.072"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300970"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AY42244E"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.05.028"},{"key":"e_1_2_8_36_1","first-page":"245","article-title":"Aflatoxin B1 in eggs and chicken livers by dispersive liquid\u2013liquid microextraction and HPLC","volume":"8","author":"Amirkhizi B.","year":"2015","journal-title":"Food Addit. Contam. Part B"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.09.035"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24061029"},{"key":"e_1_2_8_39_1","unstructured":"ChemSpider Search and share chemistry websitehttp:\/\/www.chemspider.com. Accessed January 9 2020."},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.08.042"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.05.051"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.04.066"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201901129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.201901129","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201901129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:59:45Z","timestamp":1760867985000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201901129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,24]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/jssc.201901129"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201901129","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,24]]},"assertion":[{"value":"2019-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}