{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:31:09Z","timestamp":1760920269037,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phenyl myristate was isolated from <jats:italic>Homalium nepalense<\/jats:italic>, which is known for its therapeutic virtues in traditional medicine. However, the study of radical scavenging\u2010capacity of phenyl myristate is limited by its relatively low abundance in medicinal plants. We have studied the isolation, structure\u2010elucidation, and bioactivities of high\u2010performance thin\u2010layer chromatography validated phenyl myristate from hydroalcohol\u2010extract of bark of <jats:italic>H. nepalense<\/jats:italic>. The chemical structure of phenyl myristate was elucidated by spectroscopic methods. The chromatography was performed on high\u2010performance thin\u2010layer chromatography aluminum plates coated with silica\u2010gel 60 F<jats:sub>254<\/jats:sub>. Determination and quantitation of phenyl myristate were performed by densitometric\u2010scanning at 254\u00a0nm (chloroform\u2010methanol, 9:1, v\/v; R<jats:italic><jats:sub>f<\/jats:sub><\/jats:italic> 0.49). The method was validated according to International Council for Harmonisation guidelines in terms of linearity, specificity, sensitivity, accuracy, precision, robustness, and stability. Linearity\u2010range of phenyl myristate was 100\u2013500\u00a0ng\/5\u00a0\u00b5L with correlation\u2010coefficient <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.9997. Limits of detection and quantitation were 3.35 and 10.17\u00a0ng, respectively. Phenyl myristate showed significant free\u2010radical\u2010scavenging activities in 2,2\u02d7diphenyl\u02d71\u02d7picrylhydrazyl, oxygen\u2010radical\u2010absorbance\u2010capacity, and ex vivo cell\u2010based\u2010antioxidant\u2010protection\u2010in\u2010erythrocytes assays. Molecular\u2010docking approach of phenyl myristate showed effective binding at active sites of human serum albumin (HSA) with the lowest binding energy (\u22128.4\u00a0kcal\/mol) that was comparable with ascorbic acid (\u22125.0\u00a0kcal\/mol). These studies provide mechanistic insight into the potential free radical scavenging activities of phenyl myristate.<\/jats:p>","DOI":"10.1002\/jssc.201901178","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T13:24:45Z","timestamp":1581600285000},"page":"1566-1575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Computational and experimental validation of free radical scavenging properties of high\u2010performance thin\u2010layer chromatography quantified phenyl myristate in <i>Homalium nepalense<\/i>"],"prefix":"10.1002","volume":"43","author":[{"given":"Satish","family":"Kanhar","sequence":"first","affiliation":[{"name":"Medicinal &amp; Aromatic Plant Division, Regional Plant Resource Centre, Forest &amp; Environment Department Government of Odisha  Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Partha Pratim","family":"Roy","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical &amp; Medicinal Chemistry Institute of Pharmacy Guru Ghasidas University  Bilaspur Chhattisgarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6524-4642","authenticated-orcid":false,"given":"Atish Kumar","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Medicinal &amp; Aromatic Plant Division, Regional Plant Resource Centre, Forest &amp; Environment Department Government of Odisha  Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21050559"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(97)01048-9"},{"key":"e_1_2_8_4_1","first-page":"1","article-title":"Ethnobotany of stem bark of certain plants of Visakhapatnam district, Andhra Pradesh","volume":"2","author":"Rao J. 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