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Br. is a medicinal plant. The most important bioactive compounds of <jats:italic>M. cordata<\/jats:italic> are alkaloids that have many biological activities including antifungal, anti\u2010inflammatory, and antitumor. In this study, an ionic\u2010liquid\u2010modified high\u2010speed counter\u2010current chromatography method was established to obtain alkaloids from the fruits of <jats:italic>M. cordata<\/jats:italic>. The conditions of ionic\u2010liquid\u2010modified high\u2010speed counter\u2010current chromatography, including solvent systems, the content of ionic liquid (1\u2010butyl\u20103\u2010methylimidazolium tetrafluoroborate [C<jats:sub>4<\/jats:sub>mim][BF<jats:sub>4<\/jats:sub>]), and the posttreatment of the ionic liquid, were investigated. Five alkaloids protopine, allocryptopine, sanguinarine, 8\u2010O\u2010demethylchelerythrine, and chelerythrine were separated from the extract of the fruits using a high speed counter\u2010current chromatography with two\u2010phase solvent system composed of dichloromethane\/methanol\/0.3\u00a0mol\/L hydrochloric acid aqueous solution\/[C<jats:sub>4<\/jats:sub>mim][BF<jats:sub>4<\/jats:sub>] (4:2:2:0.015, v\/v). Their purities were 96.33, 95.56, 97.94, 96.22, and 97.90%, respectively. The results indicated that a small amount of ionic liquids as modifier of the two\u2010phase solvent system could shorten the separation time and improve the separation efficiency of the alkaloids from the fruits. The ionic\u2010liquid\u2010modified high\u2010speed counter\u2010current chromatography would provide a feasible way for highly effective separation of alkaloids from natural products.<\/jats:p>","DOI":"10.1002\/jssc.201901242","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T05:09:48Z","timestamp":1584335388000},"page":"2459-2466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Isolation and purification of alkaloids from the fruits of <i>Macleaya cordata<\/i> by ionic\u2010liquid\u2010modified high\u2010speed counter\u2010current chromatography"],"prefix":"10.1002","volume":"43","author":[{"given":"Yuanyuan","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology  Beijing P. R. 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