{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:31:50Z","timestamp":1761611510433,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, a solid\u2010phase extraction with liquid chromatography and tandem mass spectrometry method was developed to determine the degree of glycosylation of glycosylation sites and the ratio of free carrier protein to total carrier protein for glycoconjugate vaccines. To remove and enrich the glycosylated peptides, a solid\u2010phase extraction method was developed, optimized, and hyphenated to liquid chromatography\u2212tandem mass spectrometry. The developed solid\u2010phase extraction with liquid chromatography\u2212tandem mass spectrometry method was shown to possess a wide linear dynamic range (0.03\u2212100\u00a0\u03bcg\/mL), a high sensitivity (0.03\u00a0\u03bcg\/mL for CRM197), good interday and intra\u2010day precision (relative standard deviation of peak area\u00a0&lt;\u00a03.3%), and good recoveries from vaccine matrix (90\u2212105%). Finally, the method was utilized to determine the degree of glycosylation and free carrier protein to total carrier protein ratio for pneumococcal conjugate vaccines and meningococcal vaccines. For quality evaluation of glycoconjugate vaccines, the method could provide more information than the traditional size exclusion chromatography method. Fourteen and twelve reported glycosylation sites for CRM197\u2010 and tetanus toxin\u2010based vaccines can be detected, respectively.<\/jats:p>","DOI":"10.1002\/jssc.202000075","type":"journal-article","created":{"date-parts":[[2020,5,10]],"date-time":"2020-05-10T11:04:15Z","timestamp":1589108655000},"page":"2880-2888","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Determination of glycosylation degree for glycoconjugate vaccines using a solid\u2010phase extraction combined with liquid chromatography and tandem mass spectrometry method"],"prefix":"10.1002","volume":"43","author":[{"given":"Zhen","family":"Long","sequence":"first","affiliation":[{"name":"Shimadzu (China) Co. Ltd  Beijing P. R. 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