{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:14Z","timestamp":1786978154045,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2016369"],"award-info":[{"award-number":["2016369"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    An efficient combination strategy based on high\u2010speed shear dispersing emulsifier technique and high\u2010performance countercurrent chromatography was developed for on\u2010line extraction and isolation of carotenoids from the fruits of\n                    <jats:italic>Lycium barbarum<\/jats:italic>\n                    . In this work, the high\u2010speed shear dispersing emulsifier technique has been employed to extract crude extracts using the upper phase of high\u2010performance countercurrent chromatography solvent system composed of\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\u2212dichloromethane\u2212acetonitrile (10:4:6.5, v\/v) as the extraction solvent. At the separation stage, the high\u2010performance counter\u2010current chromatography process adopts elution\u2013extrusion mode and the upper phase of the solvent system as stationary phase (reverse\u2010phase mode). As a result, three compounds including zeaxanthin, zeaxanthin monopalmitate, and zeaxanthin dipalmitate with purities of 89, 90, and 93% were successfully obtained in one extraction\u2010separation operation within 120\u00a0min. The targeted compounds were analyzed and identified by high\u2010performance liquid chromatography, mass spectrometry, and NMR spectroscopy. The results indicated that the present on\u2010line combination method could serve as a simple, rapid, and effective way to achieve weak polar\u00a0and unstable compounds from natural products.\n                  <\/jats:p>","DOI":"10.1002\/jssc.202000215","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T12:23:29Z","timestamp":1588940609000},"page":"2949-2958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effective on\u2010line high\u2010speed shear dispersing emulsifier technique coupled with high\u2010performance countercurrent chromatography method for simultaneous extraction and isolation of\u00a0carotenoids from\n                    <i>Lycium barbarum<\/i>\n                    L. fruits"],"prefix":"10.1002","volume":"43","author":[{"given":"Yuan","family":"Gong","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS)  Lanzhou P. R. China"},{"name":"University  of Chinese Academy of Sciences  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-9175","authenticated-orcid":false,"given":"Xin\u2010Yi","family":"Huang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS)  Lanzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Fei","family":"Liu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS)  Lanzhou P. R. China"},{"name":"University  of Chinese Academy of Sciences  Beijing P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1472-9858","authenticated-orcid":false,"given":"Duo\u2010Long","family":"Di","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS)  Lanzhou P. R. 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