{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:05:30Z","timestamp":1791655530525,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501448"],"award-info":[{"award-number":["31501448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An effective two\u2010dimensional liquid chromatography method has been established for the analysis of all<jats:italic>\u2010trans\u2010<\/jats:italic>astaxanthin and its geometric isomers from <jats:italic>Phaffia rhodozyma<\/jats:italic> employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10\u2010port valve using the photo\u2010diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32\u20131.14% and 98.21\u2013106.13%, respectively. By using two\u2010dimensional liquid chromatography, it was found that day light, ultrasonic treatment, and heat treatment have significant influence on the content of all<jats:italic>\u2010trans<\/jats:italic>\u2010astaxanthin in the extract from <jats:italic>P. rhodozyma<\/jats:italic> due to the transformation of all<jats:italic>\u2010trans<\/jats:italic>\u2010astaxanthin to <jats:italic>cis\u2010<\/jats:italic>astaxanthin. The day light and ultrasonic treatments more likely transform all<jats:italic>\u2010trans<\/jats:italic>\u2010astaxanthin to 9<jats:italic>\u2010cis<\/jats:italic>\u2010astaxanthin, and the thermal treatment transforms all<jats:italic>\u2010trans<\/jats:italic>\u2010astaxanthin to 13<jats:italic>\u2010cis<\/jats:italic>\u2010astaxanthin. These results indicate that the two\u2010dimensional liquid chromatography method can facilitate monitoring astaxanthin isomerization in the raw extract from <jats:italic>P. rhodozyma<\/jats:italic>. In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers.<\/jats:p>","DOI":"10.1002\/jssc.202000257","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T10:23:55Z","timestamp":1591525435000},"page":"3206-3215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Two\u2010dimensional liquid chromatography analysis of all\u2010<i>trans\u2010<\/i>, 9\u2010<i>cis\u2010<\/i>, and 13\u2010<i>cis\u2010<\/i>astaxanthin in raw extracts from <i>Phaffia rhodozyma<\/i>"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8820-204X","authenticated-orcid":false,"given":"Xiping","family":"Du","sequence":"first","affiliation":[{"name":"College of Food and Biological Engineering Jimei University  Xiamen P. R. 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