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The <jats:italic>n<\/jats:italic>\u2010butanol\u2013soluble extract of cascara sagrada was separated by off\u2010line two\u2010dimensional high\u2010performance countercurrent chromatography. The first\u2010dimensional high\u2010performance countercurrent chromatography resolved the <jats:italic>n<\/jats:italic>\u2010butanol\u2010soluble extract (510\u00a0mg) of cascara sagrada using the flow\u2010rate gradient method with a chloroform\u2013methanol\u2013isopropanol\u2013water (6:6:1:4, v\/v\/v\/v, normal\u2010phase mode) system to afford four anthraquinone diglucoside fractions (groups I [cascarosides C\u2013D, 71\u00a0mg], II [cascarosides E\u2013F, 56\u00a0mg], III [cascaroside A, 53\u00a0mg], and IV [cascaroside B, 31\u00a0mg]). Groups I and II were separated by the second\u2010dimensional high\u2010performance countercurrent chromatography using an ethyl acetate\u2013<jats:italic>n<\/jats:italic>\u2010butanol\u2013water (7:3:10, v\/v\/v, normal\u2010phase mode) system to yield cascarosides C (34\u00a0mg), D (26\u00a0mg), E (19\u00a0mg), and F (15\u00a0mg). Additionally, one\u2010step preparative\u2010scale high\u2010performance countercurrent chromatography method was developed to isolate large amounts of cascarosides A (389\u00a0mg) and B (187\u00a0mg) from the water\u2010soluble extract (2.1\u00a0g) of cascara sagrada using an ethyl acetate\u2013<jats:italic>n<\/jats:italic>\u2010butanol\u2013water (2:8:10, v\/v\/v, normal\u2010phase mode) system. The current study demonstrated that high\u2010performance countercurrent chromatography is a powerful technique for the isolation of marker compounds from herbal materials.<\/jats:p>","DOI":"10.1002\/jssc.202000597","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T08:04:51Z","timestamp":1599033891000},"page":"4036-4046","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation of six anthraquinone diglucosides from cascara sagrada bark by high\u2010performance countercurrent chromatography"],"prefix":"10.1002","volume":"43","author":[{"given":"Taewoong","family":"Rho","sequence":"first","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyn Woo","family":"Kil","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ju","family":"Seo","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kye Jung","family":"Shin","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences The Catholic University of Korea  Bucheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2156-4548","authenticated-orcid":false,"given":"Daijie","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Academy of Sciences Shandong Analysis and Test Center  Shandong P. 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