{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T21:09:09Z","timestamp":1761340149804,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Danqi tablet composed of the dried roots of <jats:italic>Salvia miltiorrhiza<\/jats:italic> and <jats:italic>Panax notoginseng<\/jats:italic> is a well\u2010known Chinese patent medicine commonly used for the treatment of cardio\u2010cerebrovascular diseases such as coronary heart disease and myocardial ischemia. Numerous chemical constituents belonging to <jats:italic>S. miltiorrhiza<\/jats:italic> and <jats:italic>P. notoginseng<\/jats:italic> were detectable in Danqi tablet. Here, we established and validated a rapid and sensitive ultra\u2010performance liquid chromatography coupled with triple quadrupole mass spectrometry method for simultaneous quantification of 23 components in Danqi tablet and then successfully applied to assay 12 batches of samples from ten manufacturers. Our results demonstrated that the contents of 23 components in 12 batches of Danqi tablets varied significantly and their quality indeed existed differently based on the principal component analysis. According to the quantitative data and the loading plot of principal component analysis, five abundant compounds in Danqi tablet were selected as characteristic chemical markers possibly responsible for the quality assessment. Among them, salvianolic acid B and ginsenoside Rg<jats:sub>1<\/jats:sub> were further chosen to be combined at 2:5 ratio to evaluate the anti\u2010thrombotic activity on phenylhydrazine\u2010induced zebrafish heart thrombosis model. Expectedly, this component combination increased the heart red blood cells intensity compared with the model group and the median effective concentration was 123.4\u00a0\u00b5g\/mL, suggestive of its well anti\u2011thrombotic effect. This study contributed to the quantitative evaluation of Danqi tablet and indicated the combination of salvianolic acid B and ginsenoside Rg<jats:sub>1<\/jats:sub> may be capable of reflecting the effect of Danqi tablet, thereby providing a reference for further investigations on the improvement of quality control and clinical application of Danqi tablet.<\/jats:p>","DOI":"10.1002\/jssc.202000932","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T13:08:58Z","timestamp":1611234538000},"page":"1552-1563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Quantitative evaluation of Danqi tablet by ultra\u2010performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay"],"prefix":"10.1002","volume":"44","author":[{"given":"Rui\u2010Jia","family":"Fu","sequence":"first","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Xiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Juan","family":"Tao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2097-223X","authenticated-orcid":false,"given":"Shi\u2010Jun","family":"Yue","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Ping","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility and State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation) and Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Shaanxi University of Chinese Medicine  Xi\u2032an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"volume-title":"Pharmacopoeia of the People's Republic of China","year":"2015","key":"e_1_2_9_2_1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(15)30002-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666161010105242"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.10.019"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1592\/phco.21.9.773.34561"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.05.011"},{"journal-title":"Chin J Integr Med","article-title":"Danqi tablet regulates energy metabolism in ischemic heart rat model through AMPK\/SIRT1\u2010PGC\u20101\u03b1 pathway","author":"Meng H.","key":"e_1_2_9_8_1"},{"key":"e_1_2_9_9_1","first-page":"36","article-title":"Influence of Danqi pills on blood lipid metabolism in patients with coronary heart disease","volume":"24","author":"Long J.","year":"2015","journal-title":"China Pharmaceuticals"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.02.035"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2016.1263"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.11.005"},{"key":"e_1_2_9_13_1","first-page":"83","article-title":"Study on compatibility of Radix et Rhizoma Salviae miltiorrhizae and Radix et Rhizoma notoginseng","volume":"15","author":"Zheng Q.","year":"2009","journal-title":"Chin J Exp Trad Med Formulae"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201801246"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-1013-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.01.023"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901462s"},{"key":"e_1_2_9_18_1","first-page":"1487","article-title":"Multi\u2010ingredient traditional Chinese medicines preparations and their effective fractions","volume":"20","author":"Ye Z.","year":"2011","journal-title":"Chin New Drug J"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4MB00529E"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135435"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.12.040"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306823"},{"key":"e_1_2_9_23_1","first-page":"CD005158","article-title":"Clopidogrel plus aspirin versus aspirin alone for preventing cardiovascular events","volume":"12","author":"Squizzato A.","year":"2017","journal-title":"Cochrane Database Syst Rev"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202000932","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202000932","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202000932","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:18:20Z","timestamp":1760879900000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202000932"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jssc.202000932"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202000932","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2021,2,2]]},"assertion":[{"value":"2020-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}