{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:15:33Z","timestamp":1772882133901,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypocrellins are anthraquinone that can act as excellent photosensitizers for photodynamic therapy. In the present work, we found that high\u2010speed countercurrent chromatography using cupric chloride as a complexing agent effectively separated hypocrellins from <jats:italic>Shiraia bambusicola<\/jats:italic> extract. The optimal two\u2010phase solvent system consisted of petroleum ether\/ethyl acetate\/methanol\/water (7:3:5.5:4.5, v\/v\/v\/v), with 0.01\u00a0mol\/L cupric chloride in the lower phase at pH of 2.45. This lower phase served as the mobile phase, whereas the upper phase acted as the stationary phase. Employing a continuous separation mode, three continuous injections were found to allow the purification of 1.2\u00a0g of crude extract in approximately 12 h. Hypocrellin B (10.8\u00a0mg), hypocrellin A (16.2\u00a0mg), and hypocrellin C (15.6\u00a0mg) were obtained from this process. Simulation of complexation of hypocrellin A with divalent copper ion by computational chemistry calculations indicated that three pairs of hydroxyl and carbonyl groups in hypocrellin A had similar binding energies, and demonstrated that hypocrellin A and B owned different metal\u2010to\u2010ligand ratios as compared to hypocrellin C. These factors could modify the partitioning of these compounds in two\u2010phase solvent system, and resulting in a suitable separation factor. This method would also be used to purify other anthraquinones from natural products.<\/jats:p>","DOI":"10.1002\/jssc.202001133","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T10:57:51Z","timestamp":1611140271000},"page":"1383-1390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Purification of hypocrellins from <i>Shiraia bambusicola<\/i> by coordinated high\u2010speed countercurrent chromatography using cupric chloride as a complexing agent"],"prefix":"10.1002","volume":"44","author":[{"given":"Hongjing","family":"Dong","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Shandong Provincial Collaborative innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanping","family":"Guo","sequence":"additional","affiliation":[{"name":"Resource Center of Chinese Materia Medica State Key Laboratory Breeding Base of Dao\u2010di Herbs China Academy of Chinese Medical Sciences  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4236-2483","authenticated-orcid":false,"given":"Xiao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"},{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"139","article-title":"Simultaneous determination three perylenequinones in Zhenjun Zhuhuang capsules by HPLC","volume":"40","author":"Zhong Q","year":"2020","journal-title":"Chin J Pharm Analy"},{"key":"e_1_2_8_3_1","first-page":"75","article-title":"Simultaneous determination of hypocrellin A, hypocrellin B and hypocrellin C by HPLC","volume":"37","author":"Kong M.","year":"2012","journal-title":"China J Chin Materia Med"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867324666170823143137"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.07.014"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2018.12.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2020.109550"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2009.02080.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-960121"},{"key":"e_1_2_8_10_1","first-page":"1006\u20131011, 1022","article-title":"Chemical constituents of Shiraia bambusicola and Hypocrella bambusae and their cytotoxic activity","volume":"31","author":"Yue Y. G.","year":"2019","journal-title":"Nat Prod Res Dev"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.460727"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.07.003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.12.006"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201901242"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.10.036"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000535"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.09.037"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.460660"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-461X(1999)75:4\/5<863::AID-QUA51>3.0.CO;2-T"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2012.03.001"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.02.011"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2009.09.020"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.07.043"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202001133","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202001133","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202001133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:18:24Z","timestamp":1760879904000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202001133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jssc.202001133"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202001133","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,2]]},"assertion":[{"value":"2020-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}