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The compatibility of Radix ginseng and Schisandra chinensis has been used in traditional Chinese medicine for treating Alzheimer's disease for many years. However, there are few studies on Radix ginseng\u2010Schisandra chinensis herb pair, and the underlying action mechanism is still unclear. In this study, the mechanism of Radix ginseng\u2010Schisandra chinensis herb pair on Alzheimer's disease was investigated by using the mass spectrometry\u2010based urinary metabolomics method. Sixteen urinary endogenous metabolites were identified as potential biomarkers. Meanwhile, 10 biomarkers were quantified with tandem mass spectrometry. The study result showed that the brain pathologic symptoms of model rats were improved and the potential biomarkers were adjusted backward significantly after the herb pair administration. The metabolic pathways linked to the herb pair\u2010regulated endogenous biomarkers included phenylalanine and tyrosine metabolism, tryptophan metabolism, purine metabolism, and so on. The above metabolic pathways reflected that Radix ginseng\u2010Schisandra chinensis herb pair mainly regulates abnormal energy metabolism, reduces inflammation, and regulates gut microbiota and neurotransmitters in the treatment of Alzheimer's disease.<\/jats:p>","DOI":"10.1002\/jssc.202100061","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T15:27:47Z","timestamp":1623338867000},"page":"3158-3166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Mass spectrometry\u2010based urinary metabolomics for exploring the treatment effects of Radix ginseng\u2010Schisandra chinensis herb pair on Alzheimer's disease in rats"],"prefix":"10.1002","volume":"44","author":[{"given":"Aimin","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry, National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry Chinese Academy of Sciences  Changchun P. 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