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Since the German kava ban has been lifted by the court, the quality evaluation is particularly important for its application, especially the flavokawains which were believed to be responsible for hepatotoxicity. Now, by employing two different standard references and four different methods to calculate the relative correction factors, eight different quantitative analyses of multicomponents by single\u2010marker methods have been developed for the simultaneous determination of eight major kavalactones and flavokawains in kava. The low standard method difference on quantitative measurement of the compounds among the external standard method and ours confirmed the reliability of the mentioned methods. A radar plot clearly illustrated that the contents of dihydrokavain and kavain were higher, whereas flavokawains A and B were lower in different kava samples. Only one of eight samples did not detect flavokawains that may be related to hepatotoxicity. In summary, by using different agents as an internal standard reference, the developed methods were believed as a powerful analytical tool not only for the qualitative and quantitative of kava constituents but also for the other multicomponents when authentic standard substances were unavailable.<\/jats:p>","DOI":"10.1002\/jssc.202100198","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T12:47:02Z","timestamp":1620305222000},"page":"2705-2716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous determination of both kavalactone and flavokawain constituents by different single\u2010marker methods in kava"],"prefix":"10.1002","volume":"44","author":[{"given":"Xiaojun","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education Ningxia Medical University  Yinchuan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education Ningxia Medical University  Yinchuan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruru","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education Ningxia Medical University  Yinchuan P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education Ningxia Medical University  Yinchuan P. R. 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