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Bip. The first novel solvent system consisted of <jats:italic>n<\/jats:italic>\u2010hexane, acetonitrile, chloroform, and water in a ratio of 5:5:1:5, which was selected for elution\u2010extrusion counter\u2010current chromatography to separate the <jats:italic>n<\/jats:italic>\u2010hexane extraction part. A total of six constituents were obtained from this part in the up phase as the stationary phase and the middle phase as the mobile phase. The second novel solvent system, composed of <jats:italic>n<\/jats:italic>\u2010hexane\u2010butyl acetate\u2010acetonitrile\u2010water (3:1:4:3, v\/v\/v\/v), was used for separating ethyl acetate extract of <jats:italic>Bidens pilosa<\/jats:italic> Linn. var. <jats:italic>radiata<\/jats:italic> Sch. Bip. Eight compounds were successfully isolated using elution\u2010extrusion counter\u2010current chromatography elution\u2010extrusion mode. Fourteen chemical constituents were identified as 2\u2010\u03b2\u2010D\u2010glucopyranosytoxy\u20101\u2010hydroxy\u20105(<jats:italic>E<\/jats:italic>)\u2010tridecene\u20107,9,11\u2010triyne (<jats:bold>Y1<\/jats:bold>), 3\u2010\u03b2\u2010D\u2010glucopyranosyloxy \u20101\u2010hydroxy\u20106(<jats:italic>E<\/jats:italic>)\u2010tetradecene\u20108,10,12\u2010triyne (<jats:bold>Y2<\/jats:bold>), 1, 2\u2010dihydroxy\u20105(<jats:italic>E<\/jats:italic>)\u2010tridecene\u20107,9, 11\u2010triyne (<jats:bold>Y3<\/jats:bold>), isorhamnetin (<jats:bold>Y4<\/jats:bold>), kaempferol (<jats:bold>Y5<\/jats:bold>), icthyothereolacetate (<jats:bold>Y6<\/jats:bold>), quercetin\u20103\u2010O\u2010\u03b2\u2010D\u2010 galactopyranosyl\u20107\u2010O\u2010\u03b2\u2010D\u2010glucopyranoside (<jats:bold>W1<\/jats:bold>), quercetin 3\u2010O\u2010\u03b2\u2010L\u2010rhamnopyranoside (<jats:bold>W2<\/jats:bold>), neosperidin dihydrochalcone (<jats:bold>W3<\/jats:bold>), quercetin (<jats:bold>W4<\/jats:bold>), quercetagetin\u20103,6,4' \u2010trimethoxyl\u2010 7\u2010O\u2010\u03b2\u2010D\u2010glucopyranoside (<jats:bold>W5<\/jats:bold>), taxifolin (<jats:bold>W6<\/jats:bold>), luteolin (<jats:bold>W7<\/jats:bold>), and apigenin (<jats:bold>W8<\/jats:bold>) by spectra of <jats:sup>1<\/jats:sup>H\u2010NMR and <jats:sup>13<\/jats:sup>C\u2010NMR data. Among them, compounds <jats:bold>Y1<\/jats:bold>, <jats:bold>Y2<\/jats:bold>, <jats:bold>Y3,<\/jats:bold> and <jats:bold>Y6<\/jats:bold> belong to polyacetylene compounds, and the rest were flavonoids. In addition, counter\u2010current chromatography has been used to separate polyacetylene compounds for the first time. All compounds in this method were isolated from <jats:italic>Bidens pilosa<\/jats:italic> Linn. var. <jats:italic>radiata<\/jats:italic> Sch. Bip. for the first time.<\/jats:p>","DOI":"10.1002\/jssc.202100330","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T13:43:01Z","timestamp":1627652581000},"page":"3540-3550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Separation of chemical constituents in <i>Bidens pilosa<\/i> Linn. var. <i>radiata<\/i> Sch. Bip. by elution\u2010extrusion counter\u2010current chromatography using two new three\u2010phase solvent systems"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6955-1204","authenticated-orcid":false,"given":"Zhenshan","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmacy Shandong University of Traditional Chinese Medicine  Jinan 250355 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhua","family":"Tian","sequence":"additional","affiliation":[{"name":"Experimental Center Shandong University of Traditional Chinese Medicine  Jinan 250355 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Shandong University of Traditional Chinese Medicine  Jinan 250355 P. R. 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