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In this study, a simple and validated high\u2010performance liquid chromatography method for the quantitation of sepiwhite was developed. Optimized forced degradation of sepiwhite at thermal, acid\/base, photolysis, oxidative, and heavy metal ions conditions were evaluated and the effect of each of them on production of specific 10%\u201330% degradants was studied by the approach of design of experiments. Sepiwhite accelerated study was conducted and toxicity of sepiwhite at each condition was tested. An optimized high\u2010performance liquid chromatography method was validated by a face\u2010centered central composition design. Ten different degradants were identified from sepiwhite and degradation behavior under different conditions was studied. Sepiwhite and its degradant products show no cytotoxicity. This optimized high\u2010performance liquid chromatography method can be applied for quality control assay and sepiwhite degradation behavior may be considered in the manufacturing of sepiwhite products.<\/jats:p>","DOI":"10.1002\/jssc.202100388","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T18:35:56Z","timestamp":1634754956000},"page":"4299-4312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Implementation of design of experiments for optimization of forced degradation conditions and development of a stability\u2010indicating high\u2010performance liquid chromatography method for sepiwhite"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7895-2157","authenticated-orcid":false,"given":"Javid","family":"Davoodi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics School of Pharmacy Mashhad University of Medical 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