{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:13:27Z","timestamp":1778688807647,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present work, a dispersive liquid\u2013liquid microextraction approach has been developed for extraction of four phytosterols (stigmasterol, \u03b2\u2010sitosterol, campesterol, and brassicasterol) from cow milk samples using organic and deep eutectic solvents and the results were critically compared. The extracted analytes were determined using high performance liquid chromatography. In the developed method, carbon tetrachloride and choline chloride:<jats:italic>p<\/jats:italic>\u2010chlorophenol deep eutectic solvent were selected to use as the best extraction solvent. Effective parameters and validation data were studied for both methods, independently. Under optimum conditions, limits of detection and quantification were within the ranges of 0.3\u20130.9 and 1.0\u20133.0\u00a0ng\/mL for organic solvent based dispersive liquid\u2013liquid microextraction and 0.09\u20130.32 and 0.3\u20131.0\u00a0ng\/mL for deep eutectic solvent based dispersive liquid\u2013liquid microextraction, respectively. Good coefficient of determinations and relative standard deviations obtained for the methods were \u22650.994 and \u22647.6%, respectively. The introduced method was performed on different milk samples for the determination of target analytes using both solvents and the results were analyzed statistically by the <jats:italic>t<\/jats:italic>\u2010test.<\/jats:p>","DOI":"10.1002\/jssc.202100395","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T21:11:01Z","timestamp":1632431461000},"page":"4167-4180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of organic and deep eutectic solvents based dispersive liquid\u2013liquid microextraction for the analysis of phytosterols in cow milk combined with high\u2010performance liquid chromatography\u2010ultraviolet detector"],"prefix":"10.1002","volume":"44","author":[{"given":"Omid","family":"Homaie","sequence":"first","affiliation":[{"name":"Drug Applied Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Student Research Committee Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1144-4523","authenticated-orcid":false,"given":"Mohammad Reza","family":"Afshar Mogaddam","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Pharmaceutical Analysis Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elnaz","family":"Tamizi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analysis Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Pharmaceutical and Food Control Department Faculty of Pharmacy, Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0458-0025","authenticated-orcid":false,"given":"Mahboob","family":"Nemati","sequence":"additional","affiliation":[{"name":"Food and Drug Safety Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Pharmaceutical and Food Control Department Faculty of Pharmacy, Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201600247"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2003.09.012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-11-1126"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2013.10.023"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<939::AID-JSFA644>3.0.CO;2-C"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(02)00006-1"},{"key":"e_1_2_9_8_1","article-title":"Dairy product oil, determination of cholesterol and phytosterols by gas chromatography","year":"2017","journal-title":"Iranian National Standardization Organization"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(02)00050-X"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.06.043"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf051505h"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf102150n"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac051211v"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.10.097"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.03.027"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(02)00006-1"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.08.029"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.04.029"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2020.104775"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201600711"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461523"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462025"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6RA20103B"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000136"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201801016"},{"key":"e_1_2_9_26_1","article-title":"Animal and vegetable fats and oils, determination of sterols and stanols in foods and dietary supplements containing added phytosterols","author":"ISO 23349","year":"2020","journal-title":"ISO"},{"key":"e_1_2_9_27_1","unstructured":"ICH.Multidisciplinary guidelines. 2021. Available from:https:\/\/www.ich.org\/page\/multidisciplinary\u2010guidelines"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.201200009"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202100395","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:51:47Z","timestamp":1760878307000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202100395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,5]]},"references-count":27,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/jssc.202100395"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202100395","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,5]]},"assertion":[{"value":"2021-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}