{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T07:12:17Z","timestamp":1776841937532,"version":"3.51.2"},"reference-count":45,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T00:00:00Z","timestamp":1631577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The global natural product\u2010based industry is growing fast with the introduction of new phytochemicals and herbal extract products from different geographical regions. <jats:italic>Swertia paniculata<\/jats:italic> is a well\u2010known plant with medicinal properties; however, the quality control for its major phytochemical constituents from the Himalayan geographical region is nevertheless reported. Therefore, the first objective of this investigation was to characterize and optimize the extraction process while the second objective was to validate a quantitative analytical method for chiratol from <jats:italic>S. paniculata<\/jats:italic> herbal extract. The chiratol was characterized with spectral analysis. The optimum extraction condition for the highest yield of metabolite was realized in chloroform as a solvent system under ultrasonication. The ultra\u2010high performance liquid chromatography coupled with photodiode array detection method for analytical quantification was validated for specificity, linearity, limits of detection, limits of quantification, precision, repeatability, recovery, and robustness using Eclipse Plus C<jats:sub>18<\/jats:sub> column (100\u00a0mm \u00d7 4.6\u00a0mm \u00d7 3.5\u00a0\u03bcm id). The gradient elution of water\/acetonitrile as mobile phase was used at a flow rate of 0.5\u00a0ml\/min. The recovery percentage was very satisfactory with values within specification. The robustness parameters showed no substantial influence of evaluated parameters by the <jats:italic>Youden<\/jats:italic> test. The developed method was ascertained to be appropriate for the proposed purpose.<\/jats:p>","DOI":"10.1002\/jssc.202100538","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T06:51:05Z","timestamp":1630392665000},"page":"3904-3913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation, optimized extraction, and ultra\u2010high performance liquid chromatography with photodiode array method for quantitative analysis of chiratol in <i>Swertia paniculata<\/i>"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2968-1706","authenticated-orcid":false,"given":"Vivek","family":"Ahluwalia","sequence":"first","affiliation":[{"name":"Bioproduct Chemistry Laboratory Center of Innovative and Applied Bioprocessing (CIAB)  Mohali India"},{"name":"Institute of Pesticide Formulation Technology (IPFT)  Gurugram 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