{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:08:42Z","timestamp":1787587722294,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present research, a blended polyacrylamide\u2010chitosan hydrogel was synthesized. For the first time, the prepared sorbent was efficiently employed in a hydrogel\u2010based spin\u2010column setup as a promising format. The proposed method was applied for monitoring the trace amounts of ketoconazole, clotrimazole, and miconazole in blood samples. Effective adsorption and desorption parameters were optimized using a central composite design and the one\u2010variable\u2010at\u2010a\u2010time method. Under the optimal conditions, the calibration curves were linear in the range of 15.0\u20131000.0, 1.0\u20131000.0, and 2.0\u20131000.0\u00a0ng mL<jats:sup>\u20131<\/jats:sup> for ketoconazole, clotrimazole, and miconazole, respectively, along with intra\u2010 and interday precision less than 8.4%. Limits of detection were obtained between 0.2 and 5.0\u00a0ng mL<jats:sup>\u20131<\/jats:sup>. The preconcentration factors were found in the range of 5.9\u20137.8. The introduced method was successfully applied for micro\u2010solid\u2010phase extraction of trace amounts of target antifungal drugs in blood samples, followed by liquid chromatography\u2010tandem mass spectrometry. Satisfactory relative recoveries of 94.5\u2013103.5% were obtained, implying method reliability. Overall, the proposed method provides good accuracy and repeatability, high reusability, and good applicability to determine antifungal drugs in complex biological matrices.<\/jats:p>","DOI":"10.1002\/jssc.202100560","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T10:49:22Z","timestamp":1635504562000},"page":"594-601","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Trace determination of antifungal drugs in biological fluids through a developed approach of hydrogel\u2010based spin\u2010column micro\u2010solid\u2010phase extraction followed by LC\u2010MS\/MS analysis"],"prefix":"10.1002","volume":"45","author":[{"given":"Mahshid","family":"Manouchehri","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemistry K.N. Toosi University of Technology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2385-7018","authenticated-orcid":false,"given":"Shahram","family":"Seidi","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemistry K.N. Toosi University of Technology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Taghi","family":"Naseri","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Sciences Tarbiat Modares University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Rouhollahi","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemistry K.N. Toosi University of Technology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"153","article-title":"A comparative study of two chromatographic techniques for the determination of group of imidazoles simultaneously","volume":"20","author":"Aldewachi HS","year":"2013","journal-title":"Int J Pharm Sci Rev Res"},{"key":"e_1_2_9_3_1","first-page":"361","article-title":"Development and validation of stability\u2010indicating method for the simultaneous determination of ketoconazole and beauvericin in pharmaceutical tablets","volume":"54","author":"Tian T","year":"2016","journal-title":"J Chromatogr Sci"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-017-3386-z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201000814"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.09.031"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-021-04758-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000660"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2019.1675699"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.04.094"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-016-0158-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AY42010H"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.15.146"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.06.036"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201000795"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400924"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2019.1579739"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NJ03482F"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.07.053"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.01.162"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.256"},{"key":"e_1_2_9_22_1","first-page":"49","volume-title":"Fundamentals and applications of chitosan","author":"Morin\u2010Crini N","year":"2019"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201301155"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3160732"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2017.06.079"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.02.143"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.07.074"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.10.071"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.11.069"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126378"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA11572D"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2010.12.026"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2016.10.037"},{"key":"e_1_2_9_34_1","first-page":"1","article-title":"A hydrogel composite prepared from alginate, an amino\u2010functionalized metal\u2010organic framework of type MIL\u2010101(Cr), and magnetite nanoparticles for magnetic solid\u2010phase extraction and UHPLC\u2010MS\/MS analysis of polar chlorophenoxy acid herbicides","volume":"186","author":"Tan SC","year":"2019","journal-title":"Microchim Acta"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2018.1503307"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(93)90122-I"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.11648\/j.ajpst.20190502.13"},{"key":"e_1_2_9_38_1","first-page":"25","article-title":"Cross\u2010linking in hydrogels\u2014a review","volume":"4","author":"Maitra J","year":"2014","journal-title":"Am J Polym Sci"},{"key":"e_1_2_9_39_1","first-page":"359","volume-title":"Validation of chromatographic methods of analysis: application for drugs that derived from herbs","author":"Indrayanto G","year":"2018"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100560","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202100560","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100560","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:07:35Z","timestamp":1760879255000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202100560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,11]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/jssc.202100560"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202100560","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,11]]},"assertion":[{"value":"2021-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}