{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:43:15Z","timestamp":1761766995933,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973450","82174090"],"award-info":[{"award-number":["81973450","82174090"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20210695"],"award-info":[{"award-number":["BK20210695"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new strategy combined gold\u2010coated magnetic nanocomposites assisted enrichment with mass spectrometry was developed for the characterization of disulfide bond\u2010contained proteins from Chinese cobra (<jats:italic>Naja atra<\/jats:italic>) venom. In this work, core\u2010shell nanocomposites were synthesized by the seed\u2010mediated growth method and used for the enrichment of snake venom proteins containing disulfide bonds. A total of 3545 tryptic digested peptides derived from 96 venom proteins in <jats:italic>Naja atra<\/jats:italic> venom were identified. The venom proteins comprised 14 toxin families including three\u2010finger toxins, phospholipase A<jats:sub>2<\/jats:sub>, snake venom metalloproteinase, cobra venom factor, and so forth. Extra 16 venom proteins were detected exclusively in the nanocomposites set, among which 11 venom proteins were from the three\u2010finger toxins family. In the present study, the proposed simple and efficient protocol replaced the tedious and laborious technologies commonly used for pre\u2010separating crude snake venom, suggesting widely implementation in low\u2010abundance or trace disulfide bond\u2010contained proteins or peptides characterization.<\/jats:p>","DOI":"10.1002\/jssc.202100620","type":"journal-article","created":{"date-parts":[[2021,12,12]],"date-time":"2021-12-12T05:48:28Z","timestamp":1639288108000},"page":"812-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A strategy for the enrichment and characterization of disulfide bond\u2010contained proteins from Chinese cobra (<i>Naja atra<\/i>) venom"],"prefix":"10.1002","volume":"45","author":[{"given":"Yuanqing","family":"Wei","sequence":"first","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Key Laboratory of Research and Development in Marine Bio\u2010resource Pharmaceutics Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binru","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilin","family":"Song","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengsong","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Qimen Institute of Snakebite  Huangshan 245000 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Anhui Qimen Institute of Snakebite  Huangshan 245000 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Xu","sequence":"additional","affiliation":[{"name":"Anhui Qimen Institute of Snakebite  Huangshan 245000 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumei","family":"Chi","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010ao","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuying","family":"Han","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9689-7283","authenticated-orcid":false,"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine  Nanjing 210023 P. R. China"},{"name":"Jiangsu Key Laboratory of Research and Development in Marine Bio\u2010resource Pharmaceutics Nanjing University of Chinese Medicine  Nanjing 210023 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.02.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.02.028"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25955-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.05.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.04.018"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40409-017-0116-9"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.12.012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.02.012"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.01.018"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006138"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.12.018"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2015.03.001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2019.103463"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2019.103418"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.02.018"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13975"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4331\/wjbc.v10.i1.17"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.04.007"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201901038"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000938"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202001153"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202001026"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac049787s"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200901566"},{"key":"e_1_2_9_26_1","first-page":"396","article-title":"Enrichment of thiol\u2010containing peptides in water buffalo horn by core\u2010shell Fe3O4@PDA@Au nano\u2010materials","volume":"36","author":"Liu T","year":"2020","journal-title":"J Nanjing Univ Tradit Chin Med."},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5TC01839K"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/la015561y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3PY01503C"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.002"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2012.08.005"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101334"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u201094\u2010007\u20106648\u20108_19\u20101"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11020095"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90299-1"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(96)00115-8"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00216-1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00848"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2018.1538799"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.06.003"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100620","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202100620","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100620","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:05:18Z","timestamp":1760882718000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202100620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,21]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/jssc.202100620"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202100620","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2021,12,21]]},"assertion":[{"value":"2021-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}