{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:59:17Z","timestamp":1764784757972,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Levetiracetam is an antiepileptic drug that is primarily approved by the Food and Drug Administration for the treatment of focal and generalized seizures. This study describes the development and validation of a highly selective and sensitive liquid chromatography\u2010tandem mass spectrometry method with triple\u2010stage fragmentation to determine levetiracetam in epileptic patient serum. After simple protein precipitation, the analytes were separated on a short reversed\u2010phase column (Agilent Poroshell 120 SB\u2010C18 column, 4.6 \u00d7 50\u00a0mm, 2.7\u00a0\u03bcm) using isocratic elution with 25% 0.1% formic acid in water (solvent A) and 75% methanol (solvent B) at a flow rate of 0.8\u00a0ml\/min. The linear range is 0.5\u201350 \u03bcg\/mL (<jats:italic>R<\/jats:italic><jats:sup>2\u00a0<\/jats:sup>&gt;\u00a00.99). All the validation data, such as lower limit of quantification, linearity, specificity, recoveries, matrix effects, and other parameters, fit the request of biological method validation guidance. Passing\u2013Bablok regression coefficients demonstrated that there is no constant bias and no proportional bias between the liquid chromatography\u2010tandem mass spectrometry methods with triple\u2010stage fragmentation and liquid multiple reaction monitoring. Bland\u2013Altman plot showed that the developed liquid chromatography\u2010tandem mass spectrometry method with triple\u2010stage fragmentation method is reliable and accurate to determine levetiracetam in human serum.<\/jats:p>","DOI":"10.1002\/jssc.202100639","type":"journal-article","created":{"date-parts":[[2021,10,16]],"date-time":"2021-10-16T16:03:24Z","timestamp":1634400204000},"page":"4255-4263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development and validation of a liquid chromatography\u2010tandem mass spectrometry method with triple\u2010stage fragmentation to determine levetiracetam in epileptic patient serum and its application in therapeutic drug monitoring"],"prefix":"10.1002","volume":"44","author":[{"given":"Zhengchao","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine the First Hospital of Jilin University  Changchun P. R. 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