{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:40:15Z","timestamp":1791567615744,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,19]],"date-time":"2021-12-19T00:00:00Z","timestamp":1639872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803713"],"award-info":[{"award-number":["81803713"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fraxini Cortex has a long history of being used as a medicinal plant in traditional Chinese medicine. However, it is challenging to differentiate and make quality evaluations for Fraxini Cortex from different origins due to their similarities in morphological features, as well as general chemical composition using traditional chemical analytical methods. In this study, a simple and effective method was developed to identify Fraxini Cortex from different origins by multi\u2010mode fingerprint combined with chemometrics. Digital images of the high\u2010performance thin\u2010layer chromatography profiles were converted to grayscale intensity, and the common patterns of high\u2010performance thin\u2010layer chromatography fingerprints were generated with ChemPattern software. Authentication and quality assessment were analyzed by similarity analysis, hierarchical cluster analysis, principal component analysis, and multivariate analysis of variance. The ultra\u2010high\u2010performance liquid chromatography fingerprints were analyzed by similarity analysis, principal component analysis, and orthogonal partial least square\u2010discriminant analysis. When combined with chemometrics, high\u2010performance thin\u2010layer chromatography and ultra\u2010high\u2010performance liquid chromatography fingerprint provided a simple and effective method to evaluate the comprehensive quality of Fraxini Cortex, and to distinguish its two original medicinal materials (<jats:italic>Fraxinus chinensis<\/jats:italic> Roxb. and <jats:italic>Fraxinus rhynchophylla<\/jats:italic> Hance.) recorded in the Chinese Pharmacopeia and its three adulterants (<jats:italic>Fraxinus mandschurica<\/jats:italic> Rupr., <jats:italic>Fraxinus pennsylvanica<\/jats:italic> Marsh., and <jats:italic>Juglans mandshurica<\/jats:italic> Maxim.). A similar workflow may be applied to establish a differentiation method for other medicinal and economic plants.<\/jats:p>","DOI":"10.1002\/jssc.202100784","type":"journal-article","created":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T13:08:10Z","timestamp":1639228090000},"page":"788-803","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A simple and effective method for identification of Fraxini Cortex from different sources by multi\u2010mode fingerprint combined with chemometrics"],"prefix":"10.1002","volume":"45","author":[{"given":"Huali","family":"Long","sequence":"first","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yao","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuai","family":"Tian","sequence":"additional","affiliation":[{"name":"Shanghai Standard Technology Co., Ltd.  Shanghai 201314 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjun","family":"Hou","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Lei","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0223-9448","authenticated-orcid":false,"given":"Dean","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9549-6448","authenticated-orcid":false,"given":"Wanying","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,19]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"232","article-title":"Study on resources of Fraxini Cortex","volume":"8","author":"Wu J","year":"1993","journal-title":"West Chin J Pharm Sci"},{"key":"e_1_2_9_3_1","first-page":"239","article-title":"Research progresson medicinal plants of Chinese fraxinus species","volume":"20","author":"Wang F","year":"2018","journal-title":"Mod Chin Med"},{"key":"e_1_2_9_4_1","first-page":"18","article-title":"A clinical study of the glycosides from Fraxinus","volume":"43","author":"Watanabe C","year":"1974","journal-title":"Folia Pharmacol Japon"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.03.043"},{"key":"e_1_2_9_6_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"key":"e_1_2_9_7_1","volume-title":"Raw 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fingerprint of Cortex Fraxini and its confusable species","volume":"40","author":"Zhang J","year":"2019","journal-title":"J Hebei Med Univ"},{"key":"e_1_2_9_29_1","first-page":"191","article-title":"Study on the chemical constituents of the seeds of Fraxinus americana","volume":"39","author":"Xiao Z","year":"2008","journal-title":"Chin Tradit Herb Drugs"},{"key":"e_1_2_9_30_1","volume-title":"Modern Chinese materia medica[M]","author":"Xiao P","year":"2002"},{"key":"e_1_2_9_31_1","first-page":"60","article-title":"Impact of tree ages on the content of aesculin,esculetin and fraxetin from barks of Fraxinus paxiana Lingelsh","volume":"14","author":"Li D","year":"2016","journal-title":"Cent South Pharm"}],"container-title":["Journal of Separation 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