{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:09:13Z","timestamp":1773281353804,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T00:00:00Z","timestamp":1646956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:p>Tolperisone and etodolac were proven to have synergistic effect for patients of acute low back pain associated with musculoskeletal spasm. In this work, a specific, highly sensitive and reproducible analytical method was developed and validated for the simultaneous determination of tolperisone and etodolac in human plasma using liquid chromatography\u2010tandem mass spectrometric technique. Liquid\u2013liquid extraction was optimized for sample preparation. Zorbax C<jats:sub>8<\/jats:sub> column (3.5\u00a0\u03bcm, 50 \u00d7 4.6\u00a0mm) was used, carrying a mobile phase mixture of 10.0\u00a0mM ammonium formate:acetonitrile (40:60, v\/v) pH 3.8, running in an isocratic mode. Chlorzoxazone acted as an internal standard. Sample volume of injection was 5.0 \u03bcL, and analysis was achieved within 2.5\u00a0min. Detection and quantitation were performed by electrospray ionization mass spectrometry using the multiple\u2010reaction monitoring mode. The proposed method could determine the analytes in the range of concentration 0.5\u2013200.0\u00a0ng mL<jats:sup>\u22121<\/jats:sup> for tolperisone and 0.05\u201320.0\u00a0\u03bcg mL<jats:sup>\u22121<\/jats:sup> for etodolac. Findings of inter\u2010 and intraday precisions were \u226412.3% with accuracy of \u00b15.0%. Pharmacokinetics study for the two drugs after oral administration of healthy human volunteers was achieved with the aid of application of the developed study.<\/jats:p>","DOI":"10.1002\/jssc.202100991","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T03:04:38Z","timestamp":1646190278000},"page":"1646-1655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sensitive and selective LC\u2010MS\/MS for the determination of tolperisone and etodolac in human plasma and application to a pharmacokinetic study"],"prefix":"10.1002","volume":"45","author":[{"given":"Marwa Ibrahim","family":"Helmy","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department Faculty of Pharmacy Helwan University  Helwan Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samah Sabry","family":"Saad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Pharmaceutical Sciences and Drug Manufacturing college Misr University for Science and Technology  6th of October City Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha Abdelmonem","family":"Hegazy","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8672-3848","authenticated-orcid":false,"given":"Ahmed Salah","family":"Fayed","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874104501408010017"},{"key":"e_1_2_9_3_1","first-page":"996","article-title":"Pharmacokinetic difference between S\u2010(+)\u2010and R\u2010(\u2010)\u2010etodolac in rats","volume":"25","author":"Shi J","year":"2004","journal-title":"Acta Pharmacol Sin"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.111.187401"},{"key":"e_1_2_9_5_1","volume-title":"Martindale the complete drug reference","author":"Sweetman SC","year":"2011"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta12110"},{"key":"e_1_2_9_7_1","first-page":"1510","article-title":"A review on tolperisone centrally acting muscle relaxant and its analytical methods","volume":"6","author":"Indukala P","year":"2017","journal-title":"World J Pharm Res"},{"key":"e_1_2_9_8_1","first-page":"318","article-title":"Rapid determination of tolperisone in human plasma by reversed phase high performance liquid chromatography","volume":"5","author":"Al Dgihter S","year":"2019","journal-title":"Saudi J Med Pharm Sci"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201900649"},{"key":"e_1_2_9_10_1","first-page":"1","article-title":"Analytical method development and validation for the estimation of etodolac in bulk and tablet dosage form by using UV spectroscopy","volume":"12","author":"Sharma R","year":"2019","journal-title":"J Int Pharm Res and Inn"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5530\/ijpi.2019.2.11"},{"key":"e_1_2_9_12_1","first-page":"1601","article-title":"Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5\u2010FU in bulk and tablet dosage form","volume":"64","author":"Goel H","year":"2021","journal-title":"Egypt J Chem"},{"key":"e_1_2_9_13_1","first-page":"1151","article-title":"Estimation of tolperisone hydrochloride and etodolac in combined dosage form by UV\u2010spectrophotometric methods","volume":"4","author":"Patel M","year":"2011","journal-title":"Res J Pharm Technol"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/2229-4708.84458"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1556\/JPC.25.2012.1.15"},{"key":"e_1_2_9_16_1","first-page":"87","article-title":"Stability indicating RP\u2010HPLC method for determination and validation of tolperisone and etodolac in pharmaceutical dosage form","volume":"5","author":"Sasya A","year":"2016","journal-title":"Int J Res Pharm Nano Sci"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202001050"},{"key":"e_1_2_9_18_1","unstructured":"FDA Guidance for industry. Bioanalytical method validation. US Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CV);2013."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.10.027"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.12.009"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100991","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202100991","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202100991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:47:38Z","timestamp":1760870858000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202100991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,11]]},"references-count":19,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/jssc.202100991"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202100991","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,11]]},"assertion":[{"value":"2021-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}