{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:46:46Z","timestamp":1792100806170,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T00:00:00Z","timestamp":1654992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>Favipiravir, molnupiravir, and ritonavir have been recently approved as the first oral antivirals for treatment of SARS\u2010CoV\u20102 viral infections. Their combination was reported in several clinical studies, alternatively, to enhance the viral eradication and improve patient's recovery times and rates. Being all orally administered, therefore, the development of new sensitive and validated methodologies for their simultaneous determination is a necessitate. In the proposed research, a sensitive, selective, and simple high\u2010performance thin layer chromatography method was developed and validated for determination of favipiravir, molnupiravir, and ritonavir. Silica gel 60F254 thin layer chromatography plates were used as stationary phase for this separation using mobile phase composed of methylene chloride:ethyl acetate:methanol:25% ammonia (6:3:4:1, v\/v\/v\/v). Densitometric detection was performed at wavelength 289 nm. Peaks of favipiravir, molnupiravir, and ritonavir were resolved at retention factors 0.22, 0.42, and 0.63, respectively. The proposed method was found linear within the specified ranges of 3.75\u2013100.00 \u03bcg\/mL for molnupiravir and favipiravir, and 2.75\u2013100.00 \u03bcg\/mL for ritonavir. Limits of detection were found to be 1.12, 1.21, and 0.89 \u03bcg\/mL for favipiravir, molnupiravir, and ritonavir, respectively. This is the first method to be reported for the simultaneous determination of the cited three antiviral drugs. The method was assessed on novel greenness metrics.<\/jats:p>","DOI":"10.1002\/jssc.202200178","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T05:46:09Z","timestamp":1652852769000},"page":"2582-2590","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Highly sensitive high\u2010performance thin\u2010layer chromatography method for the simultaneous determination of molnupiravir, favipiravir, and ritonavir in pure forms and pharmaceutical formulations"],"prefix":"10.1002","volume":"45","author":[{"given":"Roshdy E.","family":"Saraya","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Port\u2010Said University  Port\u2010Said Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6417-4737","authenticated-orcid":false,"given":"Sami 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