{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T02:59:09Z","timestamp":1789441149096,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>In this study, \u03b1\u2010glucosidase was successfully immobilized on cellulose filter paper and further applied to screening inhibitors from traditional Chinese medicines combined with capillary electrophoresis analysis. For \u03b1\u2010glucosidase immobilization, a cellulose filter paper was used as the carrier and grafted with amino groups by coating chitosan, then \u03b1\u2010glucosidase was covalently bonded on the amino\u2010modified carrier via epoxy ring\u2010opening reaction using polyethylene glycol diglycidyl ether as the crosslinker. Several parameters influencing the enzyme immobilization were optimized and the optimal values were enzyme concentration of 4 U\/mL, polyethylene glycol diglycidyl ether concentration of 1.25%, chitosan concentration of 7.5\u00a0mg\/mL, immobilization pH 7.0, crosslinking time of 4 h and immobilization time of 2 h. The immobilized \u03b1\u2010glucosidase exhibited good batch\u2010to\u2010batch reproducibility (RSD\u00a0=\u00a02.1%, <jats:italic>n<\/jats:italic>\u00a0=\u00a05), excellent storage stability (73.5% of its initial activity after being stored at 4\u00b0C for 15 days), and reusability (75% of its initial activity after 10 repeated cycles). The Michaelis constant of immobilized \u03b1\u2010glucosidase and half\u2010maximal inhibitory concentration of acarbose were calculated to be 1.12\u00a0mM and 0.38 \u03bcM, respectively. Finally, the immobilized \u03b1\u2010glucosidase was used for screening inhibitors from 14 kinds of Traditional Chinese Medicine extracts, and <jats:italic>Sanguisorbae Radix<\/jats:italic> showed the strongest inhibitory effect on \u03b1\u2010glucosidase.<\/jats:p>","DOI":"10.1002\/jssc.202200232","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T03:39:38Z","timestamp":1651721978000},"page":"2724-2733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Cellulose filter paper immobilized \u03b1\u2010glucosidase and its application to screening inhibitors from traditional Chinese medicine"],"prefix":"10.1002","volume":"45","author":[{"given":"Xiao\u2010Ya","family":"Shi","sequence":"first","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou P. R. 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