{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:03:25Z","timestamp":1762254205906,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p><jats:italic>Prunella vulgaris<\/jats:italic> L. is a well\u2010known traditional Chinese medicine for blood glucose homeostasis and antioxidant potential. Ethyl acetate fraction of <jats:italic>P. vulgaris<\/jats:italic> L. demonstrated higher phenolic content (85.53 \u00b1 6.74\u00a0mg gallic acid equivalents per gram dry weight), \u03b1\u2010glucosidase inhibitory (IC<jats:sub>50<\/jats:sub>, 69.13 \u00b1 2.86 \u03bcg\/ml), and antioxidant (IC<jats:sub>50<\/jats:sub>, 8.68 \u00b1 1.01 \u03bcg\/ml) activities. However, the bioactive polyphenols responsible for the beneficial properties remain unclear. Here, bioreaction\u2013HPLC\u2013quadrupole\u2010time\u2010of\u2010flight\u2010MS\/MS method was developed for rapid, accurate, and efficient screening and identification of polyphenols with \u03b1\u2010glucosidase inhibitory and antioxidant activities from <jats:italic>P. vulgaris<\/jats:italic> L. Bioactive polyphenols can specifically bind with \u03b1\u2010glucosidase or react with 1,1\u2010diphenyl\u20102\u2010picryl\u2010hydrazyl radical, which was easily discriminated from nonactive compounds. Subsequently, 20 bioactive polyphenols (16 phenyl propionic acid derivatives and four flavonoids) were screened and identified. Furthermore, molecular docking analysis revealed that screened 20 polyphenols bind with the active sites of \u03b1\u2010glucosidase through hydrogen bonding and \u03c0\u2013\u03c0 stacking. Density functional theory calculations demonstrated their electron transport ability and chemical reactivity. The in silico analysis confirmed the screened results. In summary, this study provided a valuable strategy for rapid discovering bioactive compounds from complex natural products and offered scientific evidence for further development and application of <jats:italic>P. vulgaris<\/jats:italic> L.<\/jats:p>","DOI":"10.1002\/jssc.202200374","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T13:54:38Z","timestamp":1657634078000},"page":"3393-3403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Screening of potent <i>\u03b1<\/i>\u2010glucosidase inhibitory and antioxidant polyphenols in <i>Prunella vulgaris<\/i> L. by bioreaction\u2013HPLC\u2013quadrupole\u2010time\u2010of\u2010flight\u2010MS\/MS and in silico analysis"],"prefix":"10.1002","volume":"45","author":[{"given":"Li","family":"Cheng","sequence":"first","affiliation":[{"name":"Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education Jiangxi University of Traditional Chinese Medicine  Nanchang P. 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