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In this study, a simple and robust non\u2010derivatization gas chromatography\u2010mass spectrometry method for simultaneous determination of the above four drugs in human plasma was developed and validated for therapeutic drug monitoring purposes. This method employed benzoic acid as the internal standard with hydrochloric acid for plasma acidification and ACN for precipitate protein. The supernatant was directly injected into gas chromatography\u2010mass spectrometry for analysis. Good linearity was obtained with linear correlation coefficients of the four analytes of 0.9988\u20130.9996. Extraction recoveries of valproic acid, vigabatrin, pregabalin, and gabapentin were respectively in the ranges of 91.3%\u201394.5%, 90.0%\u201390.9%, 90.0%\u201392.1%, and 88.0%\u201392.2% with the relative standard deviation values less than 12.6%. Intra\u2010 and inter\u2010batch precision and accuracy, and stability assays were all acceptable. Taken together, the novel method developed in this study provided easy plasma pretreatment, good extraction yield, and high chromatographic resolution, which has been successfully validated through the quantification of valproic acid in the plasma of 46 patients with epilepsy.<\/jats:p>","DOI":"10.1002\/jssc.202200622","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T05:18:49Z","timestamp":1669094329000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A simple and easy non\u2010derivatization gas chromatography\u2010mass spectrometry method for simultaneous quantification of valproic acid, gabapentin, pregabalin, and vigabatrin in human plasma"],"prefix":"10.1002","volume":"46","author":[{"given":"Peng","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Pharmacy Suining Branch of the Hospital Affiliated to Xuzhou Medical University  Suining P. R. 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