{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T04:49:11Z","timestamp":1782017351451,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>This study proposed the development of a monolithic supermacroporous affinity column for direct capture of lactoperoxidase, a glycoprotein present in milk, whey, and colostrum, with several applications due to its wide antimicrobial activity. A poly(acrylamide)\u2010based cryogel was produced by radical co\u2010polymerization of monomers in frozen aqueous solution and activated with <jats:italic>p<\/jats:italic>\u2010aminobenzenesulfonamide as a ligand for specific interaction with the lactoperoxidase. The axial liquid dispersion coefficients at different liquid flow rates were determined by measuring residence time distributions using the tracer pulse\u2010response method. The axial dispersion coefficient was low and the height equivalent to theoretical plate was not dependent on the flow velocity. The adsorptive capacity of affinity cryogel was studied as a function of flow velocity and the best condition was 0.9\u00a0cm\/min. The response surface methodology was applied to optimize the capture of the enzyme, as a function of pH and salt concentration. Higher purification factor value was found at a salt concentration of 80\u00a0mmol\/L and pH of 8.0 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). There was no influence of the variables under study on the yield (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). The results indicated that affinity cryogel is a promising chromatography support for the use in high\u2010throughput one\u2010step purification of lactoperoxidase from whey.<\/jats:p>","DOI":"10.1002\/jssc.202200639","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T16:33:20Z","timestamp":1669998800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synthesis and characterization of supermacroporous cryogel with immobilized <i>p<\/i>\u2010aminobenzenesulfonamide as affinity ligand for the purification of lactoperoxidase from whey"],"prefix":"10.1002","volume":"46","author":[{"given":"K\u00e1tia Silva","family":"Maciel","sequence":"first","affiliation":[{"name":"Federal University of Vi\u00e7osa Department of Food Technology  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Chequer 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