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In the present study, we developed two fully automated quick, easy, cheap, effective, rugged, and safe extraction methods based on acetonitrile salting\u2010out assisted liquid\u2010liquid extraction (method 1) and acetonitrile salting\u2010out assisted liquid\u2010liquid extraction followed by dispersive solid\u2010phase extraction (method 2) using a commercially available automated liquid\u2010liquid extraction system. We applied these methods to the extraction of 14 psychotropic drugs (11 benzodiazepines and carbamazepine, quetiapine, and zolpidem) from whole blood samples. Both methods prior to liquid chromatography\u2013tandem mass spectrometry analysis exhibited high linearity of calibration curves (correlation coefficients,\u00a0&gt;\u00a00.9997), ppt level detection sensitivities, and satisfactory precisions (&lt; 8.6% relative standard deviation), accuracies (within\u00a0\u00b1\u00a016% relative error), and matrix effects (81\u2013111%). Method 1 provided higher recovery rates (80\u201391%) than method 2 (72\u201386%), whereas method 2 provided higher detection sensitivities (limits of detection, 0.003\u20130.094\u00a0ng\/mL) than method 1 (0.025\u20130.47\u00a0ng\/mL) owing to the effectiveness of its dispersive solid\u2010phase extraction cleanup step. These fully automated extraction methods realize reliable, labor\u2010saving, user\u2010friendly, and hygienic extraction of target analytes from whole blood samples.<\/jats:p>","DOI":"10.1002\/jssc.202200681","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T11:10:05Z","timestamp":1670411405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of two fully automated quick, easy, cheap, effective, rugged, and safe pretreatment methods for the extraction of psychotropic drugs from whole blood samples"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0342-3140","authenticated-orcid":false,"given":"Takaya","family":"Murakami","sequence":"first","affiliation":[{"name":"Forensic Science Laboratory Ishikawa Prefectural Police Headquarters  Kanazawa Japan"},{"name":"Institute of Science and Engineering Kanazawa University  Kanazawa 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