{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:41:50Z","timestamp":1760920910615,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>Solvent system selection is a crucial and the most time\u2010consuming step for successful countercurrent chromatography separation. A thin\u2010layer chromatography\u2010based generally useful estimate of solvent systems method has been developed to simplify the solvent system selection. We herein utilized the method to select a solvent system for off\u2010line two\u2010dimensional countercurrent chromatography to separate chemical compositions from a complex fraction of the <jats:italic>Siraitia grosvenorii<\/jats:italic> root extract. The first\u2010dimensional countercurrent separation using chloroform\/methanol\/water (10:5.5:4.5, v\/v\/v) yielded four compounds with high purity and three mixture fractions (Fr I, III, and VII). The second\u2010dimensional countercurrent separation conducted on Fr I, III, and VII using the hexane\/ethyl acetate\/methanol\/water (4:6:6:4, 3:7:3:7, v\/v\/v) and chloroform\/methanol\/water (10:9:6, v\/v\/v) solvent systems, respectively, produced another four compounds. Four triterpenoids and four lignans were finally isolated, including two novel compounds. Hence, the generally useful estimate of solvent systems method is a feasible and efficient approach for selecting an applicable solvent system for separating complex samples. In addition, the off\u2010line two\u2010dimensional countercurrent chromatography method can improve both the peak resolution and the capacity of countercurrent chromatography.<\/jats:p>","DOI":"10.1002\/jssc.202200708","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T23:21:12Z","timestamp":1670628072000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The generally useful estimate of solvent systems method facilitates off\u2010line two\u2010dimensional countercurrent chromatography for isolating compositions from <i>Siraitia grosvenorii<\/i> roots"],"prefix":"10.1002","volume":"46","author":[{"given":"Fenglai","family":"Lu","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization Guangxi Institute of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences  Guilin P. R. 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